Anti-ALOX15 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16050

Core Product Specifications and Parameters

Biological Background: ALOX15 Function and Localization

• ALOX15 is a non-heme iron-containing dioxygenase that stereo-specifically peroxidizes free and esterified polyunsaturated fatty acids, converting arachidonate to 12-HPETE and 15-HPETE, and linoleate to 13-HPODE (PubMed:17052953, PubMed:1944593, PubMed:24282679, PubMed:8334154). Related references: PMID:17052953 PMID:1944593 PMID:24282679 PMID:8334154
• It generates specialized pro-resolving mediators like resolvin D5 and diHPDHA from DHA, actively down-regulating immune responses and exhibiting anti-aggregation properties with platelets (PubMed:32404334). Related references: PMID:32404334
• The enzyme converts epoxy fatty acids to hydroperoxy-epoxides and then to monocyclic endoperoxides, contributing to a distinct lipid mediator pathway (PubMed:25293588). Related references: PMID:25293588
• ALOX15 is involved in the cellular response to IL-13, linking it to ER stress and inflammation in adipocytes, pancreatic islets, and liver (PubMed:21831839). Related references: PMID:21831839
• It plays a critical role in self-tolerance by peroxidizing membrane phosphatidylethanolamine, facilitating clearance of apoptotic cells and preventing autoimmunity; it also regulates actin polymerization and generates PPAR-gamma ligands (By similarity).
• In the cornea, it promotes epithelial wound healing through production of lipoxin A4 and neuroprotectin D1, underscoring anti-inflammatory and neuroprotective roles (By similarity).
• It localizes to the cytoplasm, cell membrane, and lipid droplets, consistent with its roles in lipid metabolism and signaling.
• Expressed in monocytes, eosinophils, and airway epithelial cells, highlighting its involvement in innate immune and inflammatory responses.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–230) targets the N-terminal domain, which may facilitate recognition of both full-length and potentially truncated isoforms; confirm band size (expected ~75 kDa) in WB using appropriate positive controls (e.g., human leukocyte or eosinophil lysates).
• For IHC-P and IF/ICC, validate antibody binding in tissue sections or cells known to express ALOX15, such as airway epithelium or inflammatory infiltrates; include proper fixation and antigen retrieval steps.
• Cross-reactivity with mouse and rat is reported; consider species-specific validation if using these models, as sequence conservation in the immunogen region may influence reactivity.
• In ELISA and other quantitative assays, optimize antibody dilution and use recombinant protein standards to assess sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated source for IVD raw materials, streamlining procurement from discovery to clinic. This ALOX15 antibody supports research in lipid mediator biology and inflammatory diseases. Beyond single reagents, we supply validated antibody pairs, bulk ancillary materials, and can assist in sourcing rare targets to accelerate your diagnostic and therapeutic development programs.