Anti-ALOX15 Monoclonal Antibody for WB, IHC-P, ELISA - P16050

Core Product Specifications and Parameters

Biological Background: ALOX15 Function and Localization

• ALOX15 (12/15-lipoxygenase) is a non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids, generating bioactive lipid mediators such as 12-HPETE and 15-HPETE from arachidonate. Related references: PMID:17052953 PMID:1944593 PMID:24282679
• It also peroxidizes linoleate to 13-hydroperoxyoctadecadienoate (13-HPODE) and converts epoxy fatty acids to monocyclic endoperoxides via hydroperoxy-epoxide intermediates. Related references: PMID:8334154 PMID:25293588
• Participates in the synthesis of specialized pro-resolving mediators (SPMs) such as resolvin D5 from DHA, which actively down-regulate immune responses and inhibit platelet aggregation. Related references: PMID:32404334
• Plays a role in immune self-tolerance by peroxidizing membrane phosphatidylethanolamine to signal the clearance of apoptotic cells, and contributes to epithelial wound healing in the cornea through lipoxin A4 and neuroprotectin D1 production.
• Involved in the cellular response to interleukin-13 (IL-13), linking lipoxygenase activity to cytokine signaling. Related references: PMID:21831839
• Subcellular localization includes cytosol, cell membrane, and lipid droplets, reflecting its dynamic role in lipid metabolism and signaling.
• Detected in monocytes, eosinophils, and airway epithelial cells, consistent with its involvement in innate immunity and airway inflammation.
• Multiple isoforms exist due to alternative splicing; catalytic activity requires non-heme iron and calcium binding.

Experimental Guidance and Technical Tips

• For Western blot, use reducing conditions and expect a band near 75 kDa. Validate expression in relevant human cell lines (e.g., THP-1 monocytes, A549 airway epithelial cells) or tissues expressing ALOX15.
• For immunohistochemistry (paraffin), consider antigen retrieval and use appropriate positive controls (e.g., inflamed lung or atherosclerotic lesion sections containing macrophages).
• In ELISA applications, pair with a matched detection antibody; use recombinant ALOX15 protein as a standard and include proper controls for cross-reactivity with mouse and rat samples.
• Given cross-reactivity with mouse and rat, verify specificity in each species before quantitative assays; perform sequence alignment if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including this anti-ALOX15 monoclonal antibody, to accelerate your diagnostic and research projects in lipid mediator biology and inflammation. Our offerings range from validated antibody pairs and recombinant proteins to bulk ancillary reagents, all backed by technical consultation from concept to commercialization. Whether you require custom antibody development or reliable sourcing for rare targets, CamelBio is your partner for streamlined, one-stop procurement.