Anti-ALOX12B Rabbit Polyclonal Antibody for WB, ELISA - O75342

Core Product Specifications and Parameters

Biological Background: ALOX12B Function and Localization

• ALOX12B (ARCI2, 12R-LOX) is an arachidonate 12-lipoxygenase, 12R-type, also known as epidermis-type lipoxygenase 12, belonging to the lipoxygenase family.
• Catalyzes the regio- and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids to generate lipid hydroperoxides, which are subsequently reduced to hydroxy derivatives.
  Related references:
  PMID:21558561 PMID:9618483 PMID:9837935
• In the skin, acts upstream of ALOXE3 on the linoleate moiety of esterified ω-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a critical step for covalently attaching ω-hydroxyceramides to corneocyte envelope proteins.
  Related references:
  PMID:21558561
• This enzymatic cascade is essential for forming the corneocyte lipid envelope and establishing the epidermal permeability barrier, preventing excessive trans-epidermal water loss.
  Related references:
  PMID:21558561
• May also regulate airway mucin expression, suggesting a role beyond skin.
  Related references:
  PMID:22441738
• Expressed in B-cells, hair follicles, foreskin keratinocytes, adult skin, and psoriatic tissue (UniProt tissue specificity).
• Localizes to the cytoplasm, predominantly in the perinuclear region.
• Functional keywords: Dioxygenase, iron-binding, lyase, lipid metabolism, oxidoreductase; associated with ichthyosis when mutated.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 1-250 amino acids of human ALOX12B; consider that epitopes may be partly buried if the protein adopts a folded conformation, potentially affecting detection in native conditions.
• For Western blot (WB), a band around 80 kDa is expected; validate antibody specificity using positive and negative control lysates (e.g., ALOX12B-overexpressing cells versus knockout).
• For ELISA, test multiple coating antigen concentrations and antibody dilutions to optimize signal-to-noise; recombinant ALOX12B protein can serve as a standard.
• The antibody cross-reacts with mouse and rat ALOX12B; however, sequence homology in the immunogen region should be checked, and cross-reactivity should be confirmed in the relevant species tissue.
• In skin research, ALOX12B expression is highest in differentiated keratinocytes; consider using epidermal equivalent models or psoriatic samples for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-ALOX12B polyclonal antibody as part of our extensive portfolio for dermatological and lipid metabolism research. We are a one-stop partner for IVD manufacturers, offering validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate your assay development. Whether you need rare‑target sourcing or full‑workflow technical consulting, CamelBio supports your journey from concept to clinic.