Anti-ALOX12 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P18054

Core Product Specifications and Parameters

Biological Background: ALOX12 Function and Localization

• ALOX12, also known as 12S-lipoxygenase or platelet-type lipoxygenase, is a polyunsaturated fatty acid lipoxygenase that catalyzes the stereospecific incorporation of molecular oxygen into fatty acids. Related references: PMID:17493578 PMID:18311922 PMID:1851637
• It primarily converts arachidonate into (12S)-HPETE, a bioactive lipid that regulates platelet activation and aggregation. Related references: PMID:17493578 PMID:22984144 PMID:24282679 PMID:8319693 PMID:8500694
• Beyond arachidonate, ALOX12 can act on other polyunsaturated fatty acids: it catalyzes epoxidation of (14S)-HPDHA to form (13S,14S)-epoxy-DHA, and it participates in sequential oxidations of DHA to produce specialized pro-resolving mediators like resolvin D5, which down-regulate immune responses. Related references: PMID:23504711 PMID:32404334
• ALOX12 transforms leukotriene A4 into lipoxin A4 and lipoxin B4, vasoactive lipids that may modulate neutrophil function. Related references: PMID:8250832
• The enzyme is implicated in tumorigenesis: it up‑regulates VEGF expression and integrin beta‑1, promoting angiogenesis, tumor cell migration, and proliferation. Related references: PMID:16638750 PMID:22237009 PMID:9751607
• ALOX12 contributes to cell survival by preventing apoptosis in vascular smooth muscle cells. Related references: PMID:23578768
• It is localized in the cytoplasm and membrane, consistent with its role in lipid mediator synthesis.
• Expressed in vascular smooth muscle cells, linking it to vascular biology and atherosclerosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 483–663 within the C‑terminal domain; the full‑length protein has a predicted weight of 76 kDa. Expect a band near 76 kDa in Western blot (WB) under reducing conditions; validate in the relevant human, mouse, or rat sample system.
• For immunofluorescence (IF‑P) and immunohistochemistry (IHC‑P), consider optimizing fixation and permeabilization protocols to preserve the cytoplasmic/membrane localization of ALOX12.
• In ELISA applications, ensure the recombinant fusion protein used as immunogen does not interfere if the assay target overlaps with that region.
• Since this is a polyclonal antibody, lot‑to‑lot consistency should be verified for critical quantitative experiments.

CamelBio: Your One‑Stop Sourcing Bridge

This anti‑ALOX12 polyclonal antibody supports research into platelet biology, inflammation, and tumor microenvironment studies. CamelBio serves as a one‑stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and laboratories with validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing. From concept to clinic, we help streamline your procurement of critical reagents for lipid signaling and vascular disease research applications.