Anti-ALKBH8 Polyclonal Antibody for WB, IHC-P, ELISA - Q96BT7

Core Product Specifications and Parameters

Biological Background: ALKBH8 Function and Localization

• ALKBH8 is a multifunctional enzyme that acts as a tRNA (carboxymethyluridine(34)-5-O)-methyltransferase and an alpha-ketoglutarate-dependent dioxygenase, also known as ABH8, TRM9, or TRMT9A.
• It catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of tRNA anticodon loops, preferentially targeting tRNA(Arg) and tRNA(Glu). Related references: PMID:20123966 PMID:20308323 PMID:31079898
• Through its dioxygenase domain, ALKBH8 hydroxylates 5-methylcarboxymethyl uridine to 5-(S)-methoxycarbonylhydroxymethyluridine, with a preference for tRNA(Gly). Related references: PMID:21285950
• The protein is required for normal survival after DNA damage and may inhibit apoptosis while promoting cell survival and angiogenesis. Related references: PMID:20308323 PMID:19293182
• ALKBH8 is localized in both the cytoplasm and nucleus, and is widely expressed with highest levels in spleen, pancreas, and lung.
• It contains iron and zinc binding sites, requires S-adenosyl-L-methionine as a methyl donor, and belongs to the AlkB family of dioxygenases.
• Mutations in ALKBH8 are associated with intellectual disability (MRT71), and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (aa 1–230) of human ALKBH8; consider verifying reactivity with specific isoforms if needed.
• For Western blot, a band around 75 kDa is expected based on the predicted molecular weight; validate in your sample lysate and optimize blocking/detection conditions.
• This antibody has been tested in IHC-P and ELISA; for new sample types or applications, perform pilot experiments and include appropriate controls.
• Cross-reactivity with human, mouse, and rat has been reported, but homology with other species should be checked if used in non-listed organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner supporting diagnostic manufacturers and research laboratories with validated antibodies, bulk ancillary reagents, and rare target sourcing. For studies in tRNA modification, epitranscriptomics, and DNA damage response, we offer a range of high-quality reagents including ALKBH8 polyclonal antibody. Leverage our integrated supply chain to accelerate your assay development from concept to clinic.