Anti-ALKBH7 Rabbit pAb for WB, ELISA - Q9BT30

Core Product Specifications and Parameters

Biological Background: ALKBH7 Function and Localization

• RNA demethylase that removes methyl groups from N(2)-dimethylguanosine and N(1)-methyladenosine in mitochondrial pre-tRNAs, critical for mitochondrial RNA processing and gene expression. Related references: PMID:34253897
• Belongs to the AlkB family of non-heme Fe(II)/α-ketoglutarate-dependent dioxygenases, functioning as both an oxidoreductase and transferase.
• Localized to the mitochondrial matrix, where it modulates mitochondrial activity through its demethylation activity.
• Required for alkylation- and oxidation-induced programmed necrosis by promoting mitochondrial membrane potential collapse and energy depletion. Related references: PMID:23666923
• Involved in fatty acid metabolism, as suggested by similarity studies.
• Widely expressed across tissues, with highest levels in pancreas, spleen, prostate, ovary, and placenta.
• Contains a mitochondrial transit peptide and binds iron as a cofactor, essential for its enzymatic function.
• Known aliases include ABH7, SPATA11, and UNQ6002.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human ALKBH7 (aa 1-221), suggesting the antibody may recognize both native and denatured forms. For WB, consider using mitochondrial fractions from human, mouse, or rat cell lysates to enrich the target. The predicted molecular weight is ~25 kDa for the precursor, but the mature mitochondrial form may be slightly smaller after processing.
• For ELISA, the antibody can be used in indirect ELISA with recombinant ALKBH7 protein. Optimize coating and detection conditions empirically.
• As a polyclonal antibody, lot-to-lot variability should be assessed. Validate specificity with appropriate controls (e.g., ALKBH7 knockdown or knockout samples).
• Shipment on ice bags to maintain stability; upon arrival, store at -20°C or lower. Avoid multiple freeze-thaw cycles by aliquoting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For research on mitochondrial RNA modifications, programmed necrosis, and fatty acid metabolism, this ALKBH7 antibody is a reliable addition to your toolkit. Contact us for custom sourcing, technical consulting, or bulk ordering to support your diagnostic development pipeline.