Anti-ALKBH5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6P6C2

Core Product Specifications and Parameters

Biological Background: ALKBH5 Function and Localization

• Functions as an RNA N6-methyladenosine (m6A) demethylase, removing m6A marks from mRNA through an oxidative mechanism that requires molecular oxygen, alpha-ketoglutarate, and iron. Related references: PMID:21264265 PMID:23177736 PMID:24489119
• m6A demethylation by ALKBH5 affects mRNA processing, translation, and nuclear export, thereby regulating gene expression. Related references: PMID:23177736 PMID:34048572 PMID:36944332
• In vitro, ALKBH5 can also demethylate N6-methyladenosine in single-stranded DNA. Related references: PMID:24616105
• Required for late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids, facilitating correct splicing and longer 3'-UTR mRNA production.
• Participates in paraspeckle assembly, a nuclear membraneless organelle, via liquid-liquid phase separation, coupled with m6A demethylation of RNAs such as NEAT1. Related references: PMID:37369679 PMID:37474102
• Acts as a negative regulator of T-cell development, inhibiting gamma-delta T-cell proliferation via JAG1 and NOTCH2 transcript demethylation, and reducing regulatory T-cell recruitment by destabilizing CCL28 mRNA.
• Predominantly localizes to nuclear speckles and the nucleoplasm.
• Widely expressed across tissues, with highest levels in lung, testis, pancreas, spleen, and ovary.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region of ALKBH5 (residues 245-394), which is well-conserved among human, mouse, and rat, consistent with cross-reactivity data.
• For WB, a band at approximately 44 kDa is expected; consider using reducing conditions and appropriate loading controls.
• For IHC-P, antigen retrieval methods and antibody dilution may require optimization; validate in tissues with known ALKBH5 expression such as testis or lung.
• For ELISA, consider using the recombinant immunogen or peptide as coating antigen for detection assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by providing validated antibody reagents against epitranscriptomic targets like ALKBH5. Our portfolio includes optimized polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents to support your immunoassay development. For custom or bulk requirements, our sourcing team can secure rare raw materials to meet your project timelines.