Anti-ALKBH5 Rabbit Monoclonal Antibody for WB, ELISA - Q6P6C2

Core Product Specifications and Parameters

Biological Background: ALKBH5 Function and Localization

• ALKBH5 catalyzes the oxidative demethylation of m6A in RNA, a reaction dependent on molecular oxygen, alpha-ketoglutarate, and iron, thereby reversing the m6A modification on mRNA.
  Related references: PMID:21264265 PMID:23177736 PMID:24489119
• Through its demethylase activity, ALKBH5 modulates mRNA processing, translation, and export, representing a critical regulator of the epitranscriptome.
  Related references: PMID:23177736 PMID:34048572 PMID:36944332
• ALKBH5 is also capable of demethylating N(6)-methyladenosine in single-stranded DNA in vitro, suggesting broader substrate potential.
  Related references: PMID:24616105
• It plays an essential role in spermatogenesis, being required for late meiotic and haploid phases; m6A demethylation by ALKBH5 in spermatocytes and round spermatids ensures correct splicing and production of longer 3'-UTR mRNAs in male germ cells.
• ALKBH5 participates in paraspeckle assembly, a nuclear membraneless organelle, through liquid-liquid phase separation, with its demethylase activity on NEAT1 non-coding RNA being integral to this process.
  Related references: PMID:37369679 PMID:37474102
• As a negative regulator of T-cell development, ALKBH5 inhibits gamma-delta T-cell proliferation by demethylating JAG1 and NOTCH2 transcripts, and suppresses regulatory T-cell recruitment via CCL28 mRNA destabilization.
• Subcellularly, ALKBH5 localizes to nuclear speckles and the nucleoplasm, consistent with its functions in RNA processing and nuclear body dynamics.
• Acetylation, phosphorylation, and ubiquitin conjugation are among the post-translational modifications annotated for ALKBH5, which may regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 66-394 of human ALKBH5) spans a large portion of the protein; consider verifying specificity in knockout or knockdown models for critical applications.
• For Western blotting, a band at approximately 44 kDa is expected; use lysates from tissues with high ALKBH5 expression (e.g., lung, testis) as positive controls.
• In ELISA, optimize coating antigen concentration and antibody dilution to achieve linear detection and minimize background.
• Due to the enzyme's nuclear localization, confirm lysis and extraction protocols are adequate when preparing lysates for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement, supplying this anti-ALKBH5 rabbit monoclonal antibody as a robust tool for RNA modification and epigenetics research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated support for sourcing rare targets to meet your assay development and manufacturing needs.