Anti-ALKBH4 Polyclonal Antibody for WB, IF/ICC - Q9NXW9

Core Product Specifications and Parameters

Biological Background: ALKBH4 Function and Localization

• ALKBH4, also known as ABH4, is an alpha-ketoglutarate-dependent dioxygenase that belongs to the AlkB family of non-heme iron proteins.
• It functions as a lysine-specific demethylase, specifically demethylating actin K84me1, which is critical for regulating actomyosin dynamics during cytokinesis and cell migration. Related references: PMID:23673617
• ALKBH4 also acts as a DNA demethylase, removing the methyl group from N6-methyladenosine (6mA) in DNA, thereby modulating Polycomb silencing and chromatin regulation.
• The protein localizes to the cytoplasm, nucleus, nucleolus, and midbody, consistent with its roles in actin dynamics and gene regulation.
• ALKBH4 is widely expressed, with highest levels in pancreas, ovary, and spleen, suggesting tissue-specific functions in these organs.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or localization.
• It is involved in transcription regulation and chromatin remodeling, linking epigenetic modifications to cytoskeletal functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ALKBH4 protein (aa 1-302), raising antibodies that recognize multiple epitopes; ideal for detecting both endogenous and overexpressed protein.
• For Western blot (WB), the predicted molecular weight is ~34 kDa; consider using appropriate blocking conditions and antibody dilution for your specific sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), ALKBH4 staining is expected in both cytoplasmic and nuclear compartments, including nucleolar and midbody localization during cell division; validate with suitable controls.
• ELISA can be used for detecting ALKBH4 in lysates or purified protein; note that the polyclonal nature may provide high sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive range of IVD raw materials, including validated antibody pairs and bulk ancillary reagents, to support diagnostic manufacturers and research labs. Our ALKBH4 antibody fits into studies of chromatin regulation and cytoskeletal dynamics, and we can assist with sourcing rare targets and custom antibody development.