Anti-ALKBH1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13686

Core Product Specifications and Parameters

Biological Background: ALKBH1 Function and Localization

• ALKBH1 is a nucleic acid dioxygenase that acts on DNA and tRNA, requiring molecular oxygen, alpha-ketoglutarate, and iron as cofactors. Related references: PMID:18603530 PMID:27497299
• It primarily functions as a tRNA demethylase, removing N1-methyladenine from various tRNAs, with preference for position 58 (m1A58) in the T-loop, thereby regulating translation initiation and elongation. Related references: PMID:27745969
• In mitochondria, ALKBH1 oxidizes mt-tRNA(Met) at cytosine(34) to 5-formylcytosine, expanding codon recognition to read AUA in addition to AUG. Related references: PMID:27497299
• ALKBH1 also demethylates N6-methyladenine in DNA, particularly at L1 repetitive elements in embryonic stem cells, contributing to silencing. Related references: PMID:30017583 PMID:30392959
• ALKBH1 exhibits DNA lyase activity, cleaving abasic sites in DNA, which generates covalent protein-DNA adducts; this activity does not require alpha-ketoglutarate. Related references: PMID:19959401 PMID:23577621
• The protein localizes to both the nucleus and mitochondria.
• ALKBH1 is ubiquitously expressed across human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110-389 of human ALKBH1, a region encompassing the catalytic domain. This design may confer strong reactivity against the full-length protein.
• For Western blot, the predicted molecular weight is 44 kDa. Additional bands may appear due to alternative splicing, post-translational modifications, or detection of homologs such as mouse Alkbh1.
• In immunofluorescence/immunocytochemistry, anticipate staining in both nuclear and mitochondrial compartments, consistent with the dual localization of ALKBH1.
• When performing immunoprecipitation, note that ALKBH1 interacts with nucleic acids; include nuclease treatment if required.
• ELISA performance should be validated using the recombinant antigen or relevant cell/tissue lysates; consider matrix effects in complex samples.
• Cross-reactivity with mouse ALKBH1 is expected, but confirm sequence identity in the immunogen region for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to high-quality IVD raw materials, supporting manufacturers and researchers in the fields of epigenetics, DNA repair, and translational control. This ALKBH1 polyclonal antibody is part of a broad portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need custom antibody development, rare target sourcing, or technical consulting, CamelBio streamlines your procurement from concept to clinic.