Anti-ALKBH1 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q13686

Core Product Specifications and Parameters

Biological Background: ALKBH1 Function and Localization

• ALKBH1 is a nucleic acid dioxygenase that acts on DNA and tRNA, requiring molecular oxygen, alpha-ketoglutarate and iron. Related references: PMID:18603530, PMID:27497299
• Primarily functions as a tRNA demethylase, removing N1-methyladenine (m1A) from tRNAs, especially at position 58 (m1A58), regulating translation initiation and elongation in response to glucose deprivation. Related references: PMID:27745969
• In mitochondria, ALKBH1 oxidizes mt-tRNA(Met) at cytosine 34 to form 5-formylcytosine (f5C), expanding codon recognition for AUA/AUG. Related references: PMID:27497299
• Demethylates DNA N6-methyladenine (m6A) at L1 elements in embryonic stem cells, potentially silencing them. Related references: PMID:30017583, PMID:30392959
• Demethylates mRNA N3-methylcytidine (m3C) modification. Related references: PMID:31188562
• Also has DNA lyase activity, introducing double-stranded breaks at abasic sites independently of alpha-ketoglutarate and iron, forming a covalent adduct. Related references: PMID:19959401, PMID:23577621
• Subcellular localization: Nucleus and mitochondria.
• Ubiquitously expressed; may play a role in placental trophoblast lineage differentiation (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), suggesting recognition of the full-length native protein and potentially certain isoforms containing this region. Validate specificity in the relevant sample system.
• For Western blotting, expect a band at approximately 44 kDa. Use appropriate loading controls and confirm cross-reactivity with human, mouse, and rat lysates.
• In immunoprecipitation (IP), pre-clearing and optimized antibody-to-lysate ratios are recommended to minimize non-specific binding.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization due to the nuclear/mitochondrial localization. Consider testing both standard citrate and EDTA-based retrieval.
• In ELISA, pair with a detection antibody recognizing a different epitope if developing a sandwich assay, and validate linearity and sensitivity with recombinant protein or peptide standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering verified antibodies like this ALKBH1 clone for DNA/RNA modification and repair research. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets, all backed by technical expertise. From concept to clinic, we accelerate your assay development with reliable supply solutions.