Anti-ALKBH1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, ELISA - Q13686

Core Product Specifications and Parameters

Biological Background: ALKBH1 Function and Localization

• ALKBH1 is a nucleic acid dioxygenase dependent on Fe(II), α-ketoglutarate, and molecular oxygen, and is the human homolog of E. coli AlkB.
  Related references: PMID:18603530, PMID:27497299
• It mediates the demethylation of N(1)-methyladenine (m1A) at position 58 of various tRNAs, regulating translation initiation and elongation in response to glucose deprivation.
  Related references: PMID:27745969
• In mitochondria, ALKBH1 specifically oxidizes 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met) to 5-formylcytosine (f5C), expanding codon recognition during mitochondrial translation.
  Related references: PMID:27497299
• ALKBH1 serves as a DNA N6-methyladenine (6mA) demethylase at L1 retrotransposons in embryonic stem cells, potentially contributing to epigenetic silencing.
  Related references: PMID:30017583, PMID:30392959
• It also demethylates N(3)-methylcytidine (m3C) in mRNA, broadening its role in RNA modification.
  Related references: PMID:31188562
• Additionally, ALKBH1 has DNA lyase activity that cleaves DNA at abasic sites independently of its dioxygenase function, generating double-strand breaks.
  Related references: PMID:19959401, PMID:23577621
• ALKBH1 localizes to both the nucleus and mitochondria, compartmentalizing its diverse nucleic acid modification functions.
• Ubiquitously expressed across human tissues, ALKBH1 may also play a role in placental trophoblast lineage differentiation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 290-389 of human ALKBH1. Alternative immunogens targeting other regions may yield antibodies with different epitope specificities.
• For Western blotting, a band corresponding to the predicted molecular weight of ~44 kDa is expected under reducing conditions. Verify band specificity using appropriate controls.
• In immunoprecipitation, the antibody can potentially capture human, mouse, and rat ALKBH1 proteins; optimize the antibody-to-lysate ratio.
• For immunofluorescence/ICC, co-localization studies with organelle-specific markers can confirm the nuclear and mitochondrial distributions of ALKBH1.
• ELISA applications may benefit from using recombinant ALKBH1 protein or peptide as the capture antigen; titrate the antibody for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this ALKBH1 rabbit monoclonal antibody as part of a comprehensive portfolio of IVD raw materials. We support diagnostic manufacturers and researchers with validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring seamless procurement from concept to clinic. Our one-stop sourcing model streamlines access to critical reagents for nucleic acid repair and tRNA modification research.