Anti-Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P05186

Core Product Specifications and Parameters

Biological Background: Alkaline Phosphatase (ALPL) Function and Localization

• Alkaline phosphatase, tissue-nonspecific isozyme (ALPL), also known as liver/bone/kidney alkaline phosphatase, is encoded by the ALPL gene and belongs to the alkaline phosphatase family.
• It functions as a hydrolase with broad substrate specificity, dephosphorylating compounds such as inorganic pyrophosphate (PPi), pyridoxal 5′-phosphate (PLP), and N-phosphocreatine. Related references: PMID:12162492, PMID:2220817
• Essential for skeletal and dental mineralization by hydrolyzing extracellular PPi, a mineralization inhibitor, to phosphate, thus promoting hydroxyapatite crystal formation. Related references: PMID:23688511, PMID:25982064
• Catalyzes the dephosphorylation of PLP to pyridoxal, the transportable form of vitamin B6, critical for neurotransmitter synthesis in the brain. Related references: PMID:20049532, PMID:2220817
• Localizes to the cell membrane via a GPI-anchor, and also associates with extracellular vesicles, mitochondrial membrane, and intermembrane space, mediating localized phosphate metabolism.
• Post-translationally modified with glycosylation, disulfide bonds, and metal binding sites for zinc and magnesium, which are essential for its catalytic activity.
• Mutations in ALPL are linked to hypophosphatasia, a metabolic bone disease, reflecting its key role in biomineralization.
• Additionally, ALPL can dephosphorylate microbial products like lipopolysaccharides (LPS), suggesting a role in modulating innate immune responses. Related references: PMID:28448526

Experimental Guidance and Technical Tips

• For Western blotting, consider a starting dilution of 1:500–1:2000 and validate in human, mouse, or rat tissue lysates that express ALPL, such as bone, liver, or kidney.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate buffer may improve signal; test in known positive tissues like osteoblasts or hepatocytes.
• In immunofluorescence (IF-P), include appropriate permeabilization and validate colocalization with membrane or extracellular vesicle markers.
• For ELISA, pair with a matched detection antibody and test with recombinant ALPL protein or biological samples containing alkaline phosphatase activity.
• The immunogen spans amino acids 120–260, lying within the catalytic domain; the antibody may recognize both membrane-bound and soluble forms.
• Due to high homology among species, cross-reactivity with mouse and rat is expected, but always validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-ALPL rabbit polyclonal antibody supports alkaline phosphatase research and assay development in mineralization, bone metabolism, and vitamin B6-related neurological studies. Contact us for custom bulk orders or technical consulting on integrating this reagent into your diagnostic workflows.