Anti-Alkaline Phosphatase (ALPL) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05186

Core Product Specifications and Parameters

Biological Background: Alkaline Phosphatase (ALPL) Function and Localization

• Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) is expressed in liver, bone, and kidney, as indicated by its alternative name.
• It catalyzes the hydrolysis of diphosphate (PPi) to phosphate, removing a potent inhibitor of mineralization, thus promoting hydroxyapatite crystal formation in bone and teeth. Related references: PMID:12162492, PMID:23688511, PMID:25982064
• ALPL dephosphorylates pyridoxal 5′-phosphate (PLP) to pyridoxal (PL), the transportable form of vitamin B6, essential for neurotransmitter synthesis in the brain. Related references: PMID:20049532, PMID:2220817
• The enzyme is localized at the cell membrane, extracellular vesicle membranes, and mitochondrial membranes, where it participates in diverse physiological processes.
• In thermogenic fat cells, mitochondrial ALPL mediates hydrolysis of N-phosphocreatine, driving a futile creatine cycle that dissipates energy as heat for adaptive thermogenesis (By similarity).
• It can dephosphorylate microbial products like lipopolysaccharides (LPS) and poly I:C, suggesting a role in modulating innate immune responses. Related references: PMID:28448526

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa1-100); this region may be buried or cleaved in mature enzyme. Confirm recognition of native protein in your experimental system.
• For Western blot (WB), a band at ~57 kDa (precursor) or ~80 kDa (mature, glycosylated) may be observed depending on sample preparation; consider using fresh lysates and protease inhibitors.
• In immunohistochemistry (IHC-P), antigen retrieval may be required for formalin-fixed paraffin-embedded sections; validate in ALPL-expressing tissues like bone, liver, or kidney.
• For ELISA applications, pairing with a detection antibody targeting a distinct epitope is recommended for sandwich format.
• Cross-reactivity with mouse and rat is confirmed; however, sequence homology should be verified if using in other species.
• Avoid multiple freeze-thaw cycles; aliquot and store at -20°C or -80°C as appropriate.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For bone biology, mineralization disorders, and thermogenesis studies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-ALPL clone, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to accelerate your assay development with reliable supply chains.