Anti-ALK Rabbit Polyclonal Antibody for WB and ELISA - Q9UM73

Core Product Specifications and Parameters

Biological Background: ALK Function and Localization

• ALK (Anaplastic lymphoma kinase) is a receptor tyrosine kinase essential for neural development, transiently expressed in specific regions of the central and peripheral nervous systems (PubMed:11121404, PubMed:11387242, PubMed:16317043). Related references: PMID:11121404 PMID:11387242 PMID:16317043
• In hypothalamic neurons, ALK acts as a key thinness protein, regulating energy expenditure by negatively controlling white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis.
• ALKAL2 ligand binding induces ALK tyrosine kinase activation and MAPK pathway signaling (PubMed:30061385, PubMed:33411331, PubMed:34646012, PubMed:34819673). ALKAL1 is not a potent physiological ligand (PubMed:34646012). Related references: PMID:30061385 PMID:33411331 PMID:34646012 PMID:34819673
• Activated ALK phosphorylates substrates including CBL, FRS2, IRS1, SHC1, and MAP kinases ERK1/2, and drives NF-κB activation via IRS1 and AKT, promoting cell survival and proliferation (PubMed:15226403, PubMed:16878150). Related references: PMID:15226403 PMID:16878150
• ALK signaling can also be modulated by pleiotrophin (PTN) and midkine (MDK), leading to anti-apoptotic and proliferative responses through MAPK and PI3K pathways (PubMed:11278720, PubMed:11809760, PubMed:12107166, PubMed:12122009). Related references: PMID:11278720 PMID:11809760 PMID:12107166 PMID:12122009
• ALK localizes to the cell membrane and is primarily expressed in the brain, CNS, small intestine, and testis, but not in normal lymphoid cells.

Experimental Guidance and Technical Tips

• The immunogen (aa 1093–1411) corresponds to the intracellular kinase domain, ensuring detection of the intracellular portion of ALK.
• For Western blotting, consider using ALK-positive cell lines (e.g., neuroblastoma) as positive controls; the predicted molecular weight is ~176 kDa, but post-translational modifications may cause band shifts.
• In ELISA applications, the antibody may serve as a capture or detection reagent; optimal coating conditions and concentrations should be validated in your assay system.
• It is recommended to empirically determine the optimal working dilution and conditions for each specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials for diagnostic manufacturers, supporting every stage from concept to clinic. For ALK-targeted research in oncology and neural development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development. Our global sourcing capabilities extend to rare targets and bulk raw materials, helping you accelerate project timelines with reliable supply.