Anti-ALK Rabbit Monoclonal Antibody for WB, ELISA - Q9UM73

Core Product Specifications and Parameters

Biological Background: ALK Function and Localization

• Functions as a neuronal receptor tyrosine kinase transiently expressed in specific CNS and PNS regions, critical for nervous system genesis and differentiation. Related references: PMID:11121404 PMID:11387242 PMID:16317043
• Acts as a key thinness protein in hypothalamic neurons, regulating energy expenditure by negatively controlling white adipose tissue lipolysis and sympathetic tone, thereby fine-tuning energy homeostasis.
• Upon binding of its ligand ALKAL2 (but not ALKAL1), ALK activates the MAPK signaling pathway, transducing extracellular signals into intracellular responses. Related references: PMID:30061385 PMID:33411331 PMID:34646012 PMID:34819673
• Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif, and induces tyrosine phosphorylation of downstream adaptors such as CBL, FRS2, IRS1, SHC1, and MAPKs ERK1/2. Related references: PMID:15226403 PMID:16878150
• Pleiotrophin (PTN) and midkine (MDK) can also regulate ALK activation: PTN triggers MAPK pathway-mediated anti-apoptotic and proliferative signals, while MDK induces phosphorylation of IRS1, MAPKs, and PI3K to promote cell proliferation. Related references: PMID:11278720 PMID:11809760 PMID:12107166 PMID:12122009
• Drives NF-kappa-B activation, likely via IRS1 and AKT, which is essential for the autocrine growth and survival signaling mediated by MDK. Related references: PMID:15226403 PMID:16878150
• Localized at the cell membrane as a single-pass transmembrane receptor, with its kinase domain inside the cell.
• Expressed in brain, CNS, small intestine, and testis, but absent in normal lymphoid cells; frequently involved in chromosomal rearrangements in cancers.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1334–1520 within the cytoplasmic kinase domain; ensure that your target epitope is preserved under your experimental conditions, especially if using truncated or denatured samples.
• For Western blot, the predicted molecular weight is approximately 176 kDa; consider using a high-molecular-weight range marker and prolonged transfer times to ensure efficient blotting of this large protein.
• When performing ELISA, appropriate controls such as recombinant ALK protein or lysates from ALK-expressing cell lines (e.g., neuroblastoma) can be used to assess specificity and sensitivity.
• Given the receptor’s documented activation by multiple ligands (ALKAL2, PTN, MDK), confirm activation status in your cell system by monitoring downstream phosphorylation events (e.g., ERK1/2 or IRS1) if you are probing signaling-competent ALK.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials and technical support. We supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for a wide range of targets, including rare and niche markers like ALK. Our comprehensive portfolio covers neuroscience, oncology, and metabolic research, ensuring you can rapidly advance from concept to clinic with reliable sourcing solutions.