Anti-ALK Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UM73

Core Product Specifications and Parameters

Biological Background: ALK Function and Localization

• ALK (Anaplastic lymphoma kinase, also known as CD246 or ALK1) is a neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems, where it plays a crucial role in the genesis and differentiation of the nervous system.
  Related references: PMID:11121404, PMID:11387242, PMID:16317043
• ALK acts as a key thinness protein: in hypothalamic neurons, it controls energy expenditure by negatively regulating white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity).
• Upon ligand binding (especially ALKAL2), ALK dimerizes and undergoes autophosphorylation on its intracellular kinase domain, triggering downstream activation of the mitogen-activated protein kinase (MAPK) pathway and other signaling cascades.
  Related references: PMID:30061385, PMID:33411331, PMID:34646012, PMID:34819673
• ALK recruits and phosphorylates the adaptor proteins IRS1, SHC1, FRS2, and CBL, leading to activation of NF-kappa-B and the AKT serine/threonine kinase, which promotes cell survival and proliferation.
  Related references: PMID:15226403, PMID:16878150, PMID:12122009
• ALK localizes to the cell membrane (UniProt annotation) and is expressed in brain, central nervous system, small intestine, and testis, but not in normal lymphoid cells.
• ALK is classified as a proto-oncogene and is associated with chromosomal rearrangements in cancer. It is a glycoprotein with a single transmembrane helix and an intracellular tyrosine kinase domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1521–1620 near the C-terminus of human ALK (NP_004295.2). For Western blotting, a band of approximately 176 kDa is expected; use appropriate molecular weight markers and validate with a known ALK-positive cell line or tissue lysate.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to detect membrane-localized ALK; consider using cell lines with confirmed ALK expression.
• In ELISA applications, the polyclonal nature may provide broad epitope coverage, but assay conditions and coating methods should be experimentally determined.
• When using mouse samples, confirm sequence homology of the immunogen region, as cross-reactivity is declared but antibody performance may vary.
• As with any polyclonal antibody, lot-to-lot variability may occur; inclusion of proper positive and negative controls is recommended for each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing platform for raw materials used in in-vitro diagnostics and research. For projects requiring anti-ALK antibodies, we provide cost-efficient access to this polyclonal antibody and can coordinate the supply of validated antibody pairs, optimized monoclonal alternatives, and ancillary reagents in bulk. Our team supports both standard procurement and the sourcing of rare or custom raw materials across neuroscience, oncology, and metabolic disease applications.