Anti-ALIX / PDCD6IP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WUM4

Core Product Specifications and Parameters

Biological Background: ALIX / PDCD6IP Function and Localization

• ALIX/PDCD6IP is a multifunctional adapter protein involved in endosomal sorting and multivesicular body (MVB) biogenesis, where it binds to lysobisphosphatidic acid (LBPA) and functions as a Class E VPS protein within the ESCRT pathway (PubMed:14739459). Related references: PMID:14739459
• It is required for cytokinesis, acting as an adapter for ESCRT-III proteins like CHMP4 to facilitate membrane fission at the midbody (PubMed:17556548, PubMed:17853893, PubMed:18641129). Related references: PMID:17556548 PMID:17853893 PMID:18641129
• ALIX regulates exosome biogenesis in cooperation with SDC1/4 and SDCBP, influencing intercellular communication (PubMed:22660413). Related references: PMID:22660413
• The protein localizes to multiple subcellular compartments including the cytoplasm, centrosome, melanosome, tight junctions, and midbody, and is also secreted in extracellular exosomes.
• ALIX undergoes post-translational modifications such as phosphorylation, acetylation, and methylation, and is linked to processes like apoptosis, cell cycle, and host-virus interactions.
• It is involved in HIV-1 budding and can functionally replace TSG101 in facilitating viral release via interaction with CHMP4B.
• Gene aliases include AIP1, ALIX, HP95, DRIP4, and MCPH29; mutations are associated with primary microcephaly (MCPH29).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–180); verify recognition of full-length protein and potential isoforms in your sample system.
• Suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; optimal dilutions should be determined experimentally.
• Expect a band around 96 kDa, but be aware that post-translational modifications or isoforms may shift the apparent molecular weight.
• Cross-reactivity with mouse and rat is noted; validate reactivity in your specific experimental context.
• For IF/ICC, use fixation and permeabilization protocols that preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated antibodies, including this anti-ALIX/PDCD6IP polyclonal antibody, ideal for studying endocytosis, apoptosis, and viral budding mechanisms. Beyond single reagents, we offer optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your IVD assay development from concept to clinic.