Anti-ALG5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y673

Core Product Specifications and Parameters

Biological Background: ALG5 Function and Localization

• ALG5 encodes dolichyl-phosphate beta-glucosyltransferase, also known as asparagine-linked glycosylation protein 5 homolog.
• It catalyzes the synthesis of dolichyl β-D-glucosyl phosphate (Dol-P-Glc), the glucose donor required for the terminal glucosylation steps in dolichol-linked oligosaccharide assembly.
• This enzyme functions in the N-glycosylation biosynthetic pathway, adding the glucose moiety that is subsequently transferred by ALG6, ALG8, and ALG10 to the growing dolichol-linked precursor oligosaccharide.
• ALG5 is an endoplasmic reticulum membrane protein with a signal-anchor topology, most likely active on the cytoplasmic side of the ER.
• Ubiquitously expressed, with highest levels in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung.
• Keywords include glycosyltransferase, alternative splicing, disease variant, and reference proteome.
• The calculated molecular weight is approximately 37 kDa (36,946 Da).

Experimental Guidance and Technical Tips

• The immunogen covers residues 34–190 of the human ALG5 protein, a region that is relatively conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, expect a band near 37 kDa; consider using appropriate positive controls (e.g., HEK293, HeLa, or liver tissue lysates) and optimizing primary antibody dilution and detection conditions.
• In IHC-P and IF/ICC, antigen retrieval (heat-induced or enzymatic) may enhance epitope accessibility in formalin-fixed samples; validate staining in tissues with known ALG5 expression such as pancreas or placenta.
• ELISA-based assays can be developed using this antibody; pairing with a standard curve of recombinant ALG5 protein is recommended for quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, from antibody pairs to ancillary reagents. This anti-ALG5 polyclonal antibody supports glycosylation and endoplasmic reticulum research, and is part of a larger portfolio of validated antibodies for N-glycan biosynthesis targets. Contact us to explore bulk ordering, custom formulations, and complementary reagent packages tailored to your assay development pipeline.