Anti-ALG2 Polyclonal Antibody for WB, ELISA - Q9H553

Core Product Specifications and Parameters

Biological Background: ALG2 Function and Localization

• ALG2, also known as alpha-1,3/1,6-mannosyltransferase ALG2, is an endoplasmic reticulum membrane protein that catalyzes the addition of the second and third mannose residues to the dolichol-linked oligosaccharide precursor for N-glycosylation.
  Related references: PMID:12684507, PMID:35136180

• It sequentially adds alpha-1,3-linked mannose followed by alpha-1,6-linked mannose to produce Man3GlcNAc(2)-PP-dolichol, the substrate for ALG11. While both linkages are possible, alpha-1,3 then alpha-1,6 is the preferred route.
  Related references: PMID:35136180

• The dolichol-linked oligosaccharide assembly begins on the cytosolic face of the endoplasmic reticulum, where ALG2 functions, and later the glycan is flipped into the ER lumen for further processing.

• ALG2 belongs to the glycosyltransferase family, and mutations in this gene have been associated with congenital disorders of glycosylation (type Ii) and congenital myasthenic syndrome.

• The protein is annotated with alternative splicing, suggesting the existence of multiple isoforms, and proteomics identification confirms its expression in human tissues.

• Keywords associated with ALG2 include transferase, transmembrane helix, and endoplasmic reticulum, reflecting its enzymatic role and membrane localization.

Experimental Guidance and Technical Tips

• The immunogen used spans a central region (aa 197–416) of human ALG2, which may provide specificity for detecting the target by WB and ELISA; cross-reactivity with mouse is expected based on supplier validation.
• For WB analysis, a band around 47 kDa is predicted; however, post-translational modifications or isoform variation could alter migration, so validate band size in your specific sample system.
• ELISA protocols may require optimization of coating antigen and antibody dilutions; consider using recombinant ALG2 protein fragments as positive controls.
• When working with mouse samples, verify antibody specificity, as sequence homology between human and mouse ALG2 is high in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B sourcing partner for diagnostic manufacturers and research labs, providing reliable access to validated raw materials such as this anti-ALG2 polyclonal antibody. Our portfolio includes optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents tailored to glycobiology and rare disease applications. Whether you need ALG2 antibodies for diagnostic assay development or other critical raw materials, CamelBio streamlines your procurement from concept to clinic.