Anti-ALG13 Rabbit Polyclonal Antibody for WB, ELISA - Q9NP73

Core Product Specifications and Parameters

Biological Background: ALG13 Function and Localization

• ALG13 is the catalytic subunit of the UDP-N-acetylglucosamine transferase complex, also known as asparagine-linked glycosylation 13 homolog or glycosyltransferase 28 domain-containing protein 1. It mediates the second step of dolichol‑linked oligosaccharide biosynthesis on the cytoplasmic face of the endoplasmic reticulum.
• Together with its obligate partner ALG14, ALG13 forms a dimeric complex that transfers a β1,4‑linked N‑acetylglucosamine (GlcNAc) from UDP‑GlcNAc to GlcNAc‑pyrophosphatedolichol, generating N,N′‑diacetylchitobiosyl diphosphodolichol. This intermediate is subsequently elongated by ALG1 and other glycosyltransferases.
• The ALG13/ALG14 complex is anchored at the endoplasmic reticulum membrane, where the dolichol‑linked oligosaccharide precursor is assembled before flipping into the ER lumen for further processing and eventual transfer to nascent polypeptides.
• Although annotated as a potential multifunctional enzyme based on sequence motifs (thiol protease, hydrolase domains), no glycosyltransferase or deubiquitinase activity has been detected for ALG13 outside its role in GlcNAc transfer. Its additional biochemical activities, if any, remain poorly characterized.
• Mutations in the ALG13 gene are associated with congenital disorder of glycosylation type Is (CDG1S) and early‑infantile epileptic encephalopathies (DEE36, EIEE36), reflecting the critical importance of this enzyme in normal neurodevelopment and global protein N‑glycosylation.
• Alternative splicing generates multiple ALG13 isoforms, and the protein is subject to ubiquitination (Ubl conjugation pathway), which may regulate its stability or function.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning residues 100–167 of human ALG13 (isoform NP_001311219.1). This epitope is conserved in mouse ALG13, supporting the observed cross‑reactivity with mouse samples.
• For Western blotting, validate antibody specificity by including positive controls such as human or mouse cell lines known to express ALG13, and consider siRNA knockdown or overexpression experiments to confirm the ~126 kDa band.
• To study the functional ALG13/ALG14 complex, consider co‑immunoprecipitation or proximity ligation assays paired with detection of both subunits.
• In ELISA applications, use recombinant ALG13 protein or the immunizing peptide as a coating antigen to establish standard curves, and titrate the antibody appropriately for optimal signal‑to‑noise ratio.
• Because ALG13 is a membrane‑associated protein, ensure lysis buffers contain adequate detergents (e.g., 1% Triton X‑100 or NP‑40) to efficiently solubilize the ER membrane fraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with quality‑assured antibodies like this anti‑ALG13 polyclonal antibody for glycobiology and CDG research. Beyond individual reagents, we support bulk procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary assay components, while also offering rare‑target custom development and technical consulting to streamline your workflow from concept to clinic.