Anti-ALG1 Rabbit Polyclonal Antibody for WB, ELISA - Q9BT22

Core Product Specifications and Parameters

Biological Background: Chitobiosyldiphosphodolichol beta-mannosyltransferase Function and Localization

• Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), also referred to as asparagine‑linked glycosylation protein 1 homolog or mannosyltransferase-1 (MT-1), is a human enzyme encoded by the ALG1 gene (aliases HMAT1, HMT1). It plays a pivotal role in the early steps of protein N‑glycosylation.
• ALG1 functions as a glycosyltransferase in the dolichol-linked oligosaccharide (DLO) biosynthetic pathway that assembles the lipid‑linked glycan precursor required for asparagine (N)‑glycosylation. This pathway commences on the cytosolic surface of the endoplasmic reticulum (ER) membrane and is completed in the ER lumen.
• Specifically, ALG1 catalyzes the transfer of the first mannose residue from GDP‑mannose to the GlcNAc₂‑PP‑dolichol intermediate on the cytoplasmic face of the ER, yielding the Man₁GlcNAc₂‑PP‑dolichol core oligosaccharide. This step is essential before subsequent mannose additions by ALG2 and other downstream enzymes.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its role in the early cytosolic phase of DLO assembly.
• ALG1 is a multi‑pass membrane protein featuring a signal‑anchor and multiple transmembrane helices. It belongs to the glycosyltransferase family and is subject to phosphorylation (UniProt keywords: Endoplasmic reticulum, Glycosyltransferase, Membrane, Signal‑anchor, Transmembrane, Transmembrane helix, Phosphoprotein).
• Alternative splicing produces several isoforms, and mutations in ALG1 are associated with congenital disorders of glycosylation (CDG1K), underscoring its crucial function in glycobiology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning residues 215‑464 of human ALG1, which covers a substantial portion of the enzyme’s cytosolic domain. This region is conserved across human, mouse, and rat, supporting the antibody’s cross‑reactivity with those species.
• For Western blot (WB), endogenous ALG1 is expected to migrate at approximately 53 kDa. The antibody may detect additional bands due to glycosylation variants or cleavage products; validation in the relevant sample system is recommended.
• This polyclonal antibody is suitable for ELISA applications. Consider using it as a capture or detection reagent after optimization with your specific assay format and antigen source.
• As a polyclonal reagent, lot‑to‑lot consistency should be verified for quantitative work. Always include appropriate positive and negative controls (e.g., cell lines with known ALG1 expression or knockout lysates).

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a one‑stop access to validated IVD raw materials, from optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. This ALG1 antibody supports investigations into N‑glycosylation pathways, congenital disorders of glycosylation, and related glyco‑biology, and is part of CamelBio’s growing portfolio of reagents for the endoplasmic reticulum and protein modification research. Partner with us to bridge your concept‑to‑clinic journey with reliable, well‑characterized biomaterials.