Anti-ALDOB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05062

Core Product Specifications and Parameters

Biological Background: ALDOB Function and Localization

• Catalyzes the aldol cleavage of fructose 1,6-bisphosphate into dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis, and the reverse stereospecific aldol addition in gluconeogenesis. Related references: PMID:10970798 PMID:12205126 PMID:20848650
• In fructolysis, metabolizes fructose 1-phosphate derived from dietary fructose into dihydroxyacetone phosphate and D-glyceraldehyde. Related references: PMID:10970798 PMID:12205126 PMID:20848650
• Acts as a tumor suppressor by stabilizing the ternary complex with G6PD and TP53, inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check. Related references: PMID:35122041
• Localizes primarily to the cytosol and is also associated with the cytoskeleton, microtubule organizing center, centrosome, and centriolar satellites.
• Known post‑translational modifications include acetylation and phosphorylation, and the enzyme forms a Schiff base intermediate during catalysis.
• The protein has a predicted molecular weight of approximately 39 kDa (UniProt: 39473 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human ALDOB, which may provide specificity for ALDOB over other aldolase isoforms.
• For Western blot, expect a band around 39 kDa; validate in the relevant sample system (e.g., liver‑derived cell lines or tissues).
• In IF/ICC, consider optimizing fixation and permeabilization conditions to capture its cytosolic and centrosomal localization.
• ELISA can be used for quantitative detection; cross‑reactivity with mouse and rat was observed, making it suitable for multi‑species studies.

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