Anti-ALDOA Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P04075

Core Product Specifications and Parameters

Biological Background: ALDOA Function and Localization

• ALDOA, or fructose-bisphosphate aldolase A, is a muscle-type aldolase belonging to the class I aldolase family.
• The enzyme catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, a pivotal reaction in glycolysis and gluconeogenesis. Related references: PMID:14766013
• Beyond its catalytic role, ALDOA functions as a scaffolding protein; upon glucose deprivation, FBP dissociation allows aldolase to promote AMPK activation via TRPV channel interaction and V-ATPase inhibition.
• The protein localizes primarily to the cytoplasm and associates with the sarcomere in muscle cells, specifically at the I band and M line.
• ALDOA undergoes various post-translational modifications, including acetylation, phosphorylation, and hydroxylation, which may influence its activity and binding partners.
• Mutations in the ALDOA gene are linked to glycogen storage disease 12 (GSD12) and hereditary hemolytic anemia, underscoring its importance in energy metabolism and red blood cell stability.
• Alternative splicing generates multiple ALDOA isoforms, potentially diversifying its functional and regulatory roles in different tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human aldolase (P04075). This N-terminal region may be conserved across species; consider performing sequence alignment to confirm epitope availability in your target samples.
• For Western blot (WB), the predicted molecular weight is 39 kDa. Use an appropriate gel percentage and verify band specificity with positive and negative controls.
• In immunohistochemistry (IHC), antigen retrieval conditions may influence outcome; optimize protocol steps such as heat-induced epitope retrieval to ensure robust staining.
• This antibody has been qualified for ELISA; consider its use in direct or sandwich formats with empirically determined coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For ALDOA-related projects in glycolysis, metabolic disorders, or oncology, we offer tailored supply solutions to support your journey from concept to clinic.