Anti-ALDOA Polyclonal Antibody for WB, IF/ICC, ELISA - P04075

Core Product Specifications and Parameters

Biological Background: ALDOA Function and Localization

• Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate into two triose phosphate molecules, a central step in glycolysis and gluconeogenesis. Related references: PMID:14766013
• In addition to its catalytic role, ALDOA acts as a scaffolding protein that, upon glucose deprivation, promotes AMPK activation by interacting with TRPV channels and inhibiting lysosomal V-ATPase (By similarity).
• ALDOA localizes to the cytoplasm and associates with the myofibril sarcomere, specifically the I band and M line, supporting energy production in muscle contraction.
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, hydroxylation, and ubiquitin-like conjugation, which may regulate its activity and stability.
• Mutations in ALDOA are associated with glycogen storage disease type 12 and hereditary hemolytic anemia, highlighting its clinical importance in metabolic and hematologic disorders.
• Alternative splicing of ALDOA generates multiple isoforms, which may contribute to tissue-specific functions and disease pathology.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1-348), which may yield antibodies recognizing multiple epitopes and native conformations, potentially enhancing signal in WB and IF/ICC.
• For Western blot, expect a band at approximately 39 kDa; validate with appropriate positive and negative controls using human, mouse, or rat cell/tissue lysates.
• In immunofluorescence/ICC, ALDOA displays a cytoplasmic and sarcomeric pattern; include appropriate fixation and permeabilization protocols to preserve antigenicity.
• For ELISA, consider pairing with a matched detection antibody and optimize coating conditions using recombinant ALDOA protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and supports diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibodies, antigens, and ancillary reagents. For glycolysis and metabolic disease research, our anti-ALDOA polyclonal antibody enables robust detection in WB, IF/ICC, and ELISA applications. We also offer customized sourcing for rare targets and bulk reagent supply, streamlining your path from concept to clinic.