Anti-ALDH7A1 Monoclonal Antibody for WB, ELISA - P49419

Core Product Specifications and Parameters

Biological Background: ALDH7A1 Function and Localization

• Alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1), also known as antiquitin-1, is a member of the aldehyde dehydrogenase family. The human ALDH7A1 gene (GeneID: 501) undergoes alternative splicing, potentially generating multiple isoforms with distinct tissue distributions and functional roles.
• Protects cells from oxidative stress by metabolizing a variety of lipid peroxidation-derived aldehydes, including 4-hydroxynonenal (4-HNE) and malondialdehyde, thus mitigating cellular damage. Related references: PMID:16491085, PMID:20207735, PMID:21338592
• Functions in cellular osmoregulation by catalyzing the conversion of betaine aldehyde to betaine, a critical osmolyte and methyl donor, thereby aiding in defense against hyperosmotic stress. Related references: PMID:20207735
• Essential for lysine catabolism in the brain, mediating the oxidation of L-aminoadipate-semialdehyde (L-AASA) to L-2-aminoadipate, linking it to neurotransmitter and metabolic pathways.
• Acts as a key inhibitor of ferroptosis through dual mechanisms: recruitment to the plasma membrane upon AMPK-dependent phosphorylation to generate NADH, supporting AIFM2/FSP1-mediated lipid peroxide reduction, and direct detoxification of reactive aldehydes like 4-HNE. Related references: PMID:40233740
• Regulates cellular energy homeostasis by inhibiting COPI-mediated intracellular transport under conditions of energy stress (e.g., starvation, hypoxia), thereby reducing energy consumption. Related references: PMID:31492851
• Displays broad subcellular distribution, localizing to mitochondria, cytoplasm, cytosol, cell membrane, Golgi apparatus membrane, and nucleus, reflecting its diverse functional roles.
• Post-translationally modified by acetylation and phosphorylation, which likely modulate its enzymatic activity, subcellular targeting, and protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human ALDH7A1, which may influence detection of specific isoforms or splice variants.
• For Western blot, denaturing conditions are recommended; validate band specificity in relevant sample systems (e.g., human or mouse cell lysates) given the predicted molecular weight of 58 kDa.
• ELISA applications may utilize either native or recombinant ALDH7A1; confirm cross-reactivity with mouse samples as indicated.
• Consider including appropriate controls for post-translational modifications if studying cellular stress or ferroptosis pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This Anti-ALDH7A1 Rabbit mAb supports research in oxidative stress, ferroptosis, cancer, and neurological disorders; CamelBio can further supply complementary reagents and custom solutions to accelerate your diagnostic assay development from concept to clinic.