Anti-ALDH6A1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q02252

Core Product Specifications and Parameters

Biological Background: ALDH6A1 Function and Localization

• Encodes methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], a mitochondrial enzyme belonging to the aldehyde dehydrogenase family 6 (ALDH6A1).
• Catalyzes the oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl-CoA and propionyl-CoA, respectively.
• Plays a central role in the catabolic pathways of valine and thymine, as well as the degradation of uracil and cytidine via the beta-alanine pathway.
• Localized within the mitochondrial matrix, consistent with its role in oxidative metabolism.
• Post-translationally modified by acetylation and phosphorylation, as identified in proteomics studies.
• Exhibits alternative splicing, with disease-associated variants recorded; classified as an NAD-dependent oxidoreductase.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 326–535, a C-terminal region of ALDH6A1. The antibody may recognize full-length protein and possibly some truncated isoforms; confirm specificity via knockdown or knockout controls.
• For Western blotting, the predicted molecular weight is approximately 58 kDa. Use reducing conditions and validate band identity in the relevant sample system.
• In immunofluorescence/immunocytochemistry, anticipate mitochondrial staining. Co-stain with a mitochondrial marker to verify localization.
• The antibody cross-reacts with human, mouse, and rat ALDH6A1. When working with rodent models, validate performance under your specific experimental conditions.
• As a polyclonal antibody, lot-to-lot consistency should be monitored. Consider including a positive control and reference standard in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ALDH6A1, a key enzyme in metabolic and mitochondrial research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whatever your target or scale, our procurement network bridges the gap between rare raw-material sourcing and reliable commercial supply.