Anti-ALDH6A1 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q02252

Core Product Specifications and Parameters

Biological Background: ALDH6A1 Function and Localization

• The ALDH6A1 gene encodes methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], a mitochondrial enzyme critical for the catabolism of valine, thymine, and beta-alanine.
• The enzyme catalyzes the NAD-dependent oxidative decarboxylation of malonate and methylmalonate semialdehydes into acetyl-CoA and propionyl-CoA, linking amino acid and nucleotide degradation to central metabolism.
• Alternative splicing of ALDH6A1 generates multiple transcript variants, though the functional significance of each isoform remains to be fully elucidated.
• Localized within the mitochondrial matrix, the protein possesses an N-terminal transit peptide that directs its import and processing in the organelle.
• Post-translational modifications including acetylation and phosphorylation have been identified, suggesting potential regulatory mechanisms of enzyme activity; mutations in ALDH6A1 are recorded as disease variants in UniProt.
• The enzyme belongs to the aldehyde dehydrogenase family and contains conserved catalytic residues typical of NAD-dependent oxidoreductases, with its 3D structure resolved.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 326–535 of human ALDH6A1, a region likely to include surface-exposed epitopes; antibody specificity should be validated in the relevant species and sample type.
• For IHC-P, consider antigen retrieval protocols and include appropriate positive (e.g., liver, kidney) and negative controls to confirm mitochondrial staining patterns.
• In IF/ICC, co-staining with a mitochondrial marker can help verify localization; fixation and permeabilization conditions may need optimization.
• ELISA applications can utilize this antibody for capture or detection; pairing with a validated matched antibody pair is advisable for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supplying high-quality antibodies and ancillary reagents for metabolic disease research. Our ALDH6A1 polyclonal antibody supports assay development for disorders of valine and thymine catabolism. Beyond individual antibodies, we offer validated antibody pairs, bulk reagent supplies, and customized sourcing solutions to accelerate your diagnostic development from concept to clinic.