Anti-ALDH5A1/SSADH Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51649

Core Product Specifications and Parameters

Biological Background: ALDH5A1 (SSADH) Function and Localization

• ALDH5A1 encodes succinate-semialdehyde dehydrogenase, mitochondrial, a member of the aldehyde dehydrogenase family.
• Catalyzes the oxidation of succinic semialdehyde to succinate, a critical step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
• Localizes primarily to mitochondria, as indicated by its mitochondrial transit peptide.
• Highly expressed in brain, pancreas, heart, liver, skeletal muscle, and kidney, with lower levels in placenta.
• Subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing produces multiple isoforms.
• Associated with disease variants, particularly in SSADH deficiency.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering the C-terminal region (aa 777-976) of human ALDH5A1. This region may be conserved across species, supporting observed cross-reactivity with mouse and rat.
• For Western blotting, consider using whole cell or mitochondrial-enriched lysates from brain, liver, or kidney tissues. Validate antibody dilution in your specific experimental system.
• For IHC-P and IF/ICC, include appropriate antigen retrieval steps and confirm signal specificity with knockdown controls if possible.
• ELISA application may require pairing with a detection antibody; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for neuroscience and metabolic research. Our ALDH5A1/SSADH antibody supports studies on GABA metabolism and related disorders. We also provide custom sourcing for rare targets, helping diagnostic manufacturers move from concept to clinic.