Anti-ALDH5A1 Rabbit Polyclonal Antibody for WB, ELISA - P51649

Core Product Specifications and Parameters

Biological Background: ALDH5A1 Function and Localization

• ALDH5A1, also known as succinate-semialdehyde dehydrogenase (SSADH) or SSDH, is a member of the aldehyde dehydrogenase family 5 subfamily A, encoded by the ALDH5A1 gene on chromosome 6.
• It catalyzes the oxidation of succinate semialdehyde to succinate, a critical step in the mitochondrial degradation pathway of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).
• This enzyme is localized to the mitochondrion, consistent with its role in central carbon and neurotransmitter metabolism, and requires NAD+ as a cofactor.
• ALDH5A1 is highly expressed in tissues active in GABA metabolism, including brain, pancreas, heart, liver, skeletal muscle, and kidney; expression is lower in placenta.
• The protein is subject to post-translational modifications such as acetylation and phosphorylation, and contains a mitochondrial transit peptide, a disulfide bond, and structural features linked to disease variants.
• Alternative splicing generates multiple transcript variants, potentially contributing to tissue-specific regulation of GABA catabolism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a region within amino acids 400–500 of human ALDH5A1; cross-reactivity with mouse and rat orthologs has been observed, but sequence alignment in the immunogen region should be verified for other species.
• For Western blot, a band around 57 kDa is expected; consider using a low-percentage gel for clear resolution and validate in the relevant sample system (e.g., brain or liver lysates).
• This antibody is suitable for ELISA; when used, consider direct coating of the peptide immunogen or optimized antigen presentation for robust signal.
• Because ALDH5A1 is a mitochondrial protein, efficient protein extraction with mitochondrial lysis buffers may enhance detection in complex tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio meets your end-to-end IVD raw-material needs with validated polyclonal antibodies like this anti-ALDH5A1 antibody, ideal for neuroscience and metabolic disorder research. Our one-stop network supports procurement of companion antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your GABA-pathway assay development. Reach out for custom logistics and technical guidance tailored to your diagnostic workflow.