Anti-ALDH4A1 Rabbit Polyclonal Antibody for WB, ELISA - P30038

Core Product Specifications and Parameters

Biological Background: ALDH4A1 Function and Localization

• ALDH4A1 (Delta-1-pyrroline-5-carboxylate dehydrogenase) is a mitochondrial matrix enzyme that catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a critical step linking the urea and tricarboxylic acid cycles.
• The preferred substrate is glutamic gamma-semialdehyde, but it also acts on succinic, glutaric, and adipic semialdehydes.
• This enzyme functions in proline and ornithine metabolism, playing a key role in amino acid catabolism and cellular energy production.
• ALDH4A1 is highly expressed in the liver, with moderate to lower expression in skeletal muscle, kidney, heart, brain, placenta, lung, and pancreas.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity; alternative splicing generates multiple transcript variants.
• Mutations in ALDH4A1 are associated with hyperprolinemia type II, a disorder of proline metabolism (disease variant).
• Keywords: 3D-structure, acetylation, alternative splicing, direct protein sequencing, disease variant, mitochondrion, NAD, oxidoreductase, phosphoprotein, proline metabolism, proteomics identification, reference proteome, transit peptide.

Experimental Guidance and Technical Tips

• The immunogen spans a region within the C-terminal half of the protein (aa 354-563); consider this epitope location for applications requiring native vs. denatured protein recognition.
• For Western blotting, expect a band near 62 kDa; include appropriate positive controls (e.g., liver or kidney lysate) and validate in your sample system.
• In ELISA, optimize coating and detection antibody concentrations; the polyclonal nature may provide high sensitivity but requires careful titration.
• Cross-reactivity with mouse is reported; if using mouse models, verify specificity using knockout tissues or siRNA knockdown.
• As a polyclonal antibody, lot-to-lot consistency may vary; we recommend routine quality checks in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your proline metabolism and mitochondrial disorder research with this high-quality anti-ALDH4A1 antibody, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. As your one-stop sourcing partner for IVD raw materials, CamelBio simplifies procurement from discovery to clinical application, also providing rare target raw-material sourcing and technical consulting.