Anti-ALDH4A1 Rabbit Monoclonal Antibody for WB - P30038

Core Product Specifications and Parameters

Biological Background: ALDH4A1 Function and Localization

• Encodes delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh), a mitochondrial matrix enzyme belonging to the aldehyde dehydrogenase family 4 member A1.
• Catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C) to glutamate, a critical step linking the urea cycle and tricarboxylic acid cycle.
• Exhibits highest activity toward glutamic gamma-semialdehyde, but also accepts succinic, glutaric, and adipic semialdehydes as substrates.
• Tissue expression is highest in liver, followed by skeletal muscle, kidney, heart, brain, placenta, lung, and pancreas.
• Post-translational modifications include acetylation and phosphorylation (based on proteomics identification).
• Alternative splicing produces multiple isoforms; classified as a disease variant gene, with potential implications in proline metabolism disorders.

Experimental Guidance and Technical Tips

• The immunogen (residues 354-563) targets a C-terminal region conserved among human, mouse, and rat, supporting cross-reactivity observations.
• For Western blotting, expect a specific band around 62 kDa under reducing conditions; validate in known positive tissues such as liver or kidney lysates.
• In immunofluorescence and immunohistochemistry, mitochondrial localization should be evident; consider co-staining with mitochondrial markers for confirmation.
• ELISA applications may be optimized using recombinant protein standards; check linearity and sensitivity for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio facilitates procurement of validated IVD raw materials, including monoclonal antibodies like this ALDH4A1 clone, for diagnostic and research applications in mitochondrial metabolism and amino acid disorders. Our supply chain covers optimized antibody pairs, bulk reagents, and rare target sourcing, supporting your development from concept to clinic.