Anti-ALDH4A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P30038

Core Product Specifications and Parameters

Biological Background: ALDH4A1 Function and Localization

• ALDH4A1, also known as delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) or aldehyde dehydrogenase family 4 member A1, is a mitochondrial enzyme.
• It catalyzes the irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C) to glutamate, linking proline and ornithine degradation to the urea cycle and tricarboxylic acid cycle.
• The enzyme exhibits substrate preference for glutamic gamma-semialdehyde but can also oxidize succinic, glutaric, and adipic semialdehydes.
• ALDH4A1 is localized to the mitochondrial matrix, where it functions in proline metabolism.
• It is highly expressed in the liver, with moderate levels in skeletal muscle, kidney, heart, brain, placenta, lung, and pancreas.
• The protein contains an N-terminal mitochondrial transit peptide and undergoes post-translational modifications such as acetylation and phosphorylation.
• Alternative splicing generates multiple transcript variants.
• Mutations in ALDH4A1 are associated with hyperprolinemia type II, an inborn error of metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 354–563 of human ALDH4A1, a C-terminal region that may provide specific detection; validate in the relevant species and sample type.
• For western blotting (WB), a band corresponding to the predicted molecular weight of approximately 62 kDa may be observed; consider using a suitable loading control and optimizing antibody dilution.
• Given the high expression in liver, tissue lysates from human, mouse, or rat liver may serve as positive controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), mitochondrial staining patterns can confirm target localization; fixation and permeabilization conditions should be tested.
• In ELISA applications, perform standard optimization of coating antigens and detection conditions.
• Cross-reactivity with human, mouse, and rat is reported; if using other species, verify sequence homology before testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from assay concept to commercialization. This ALDH4A1 polyclonal antibody is part of our portfolio for metabolic and mitochondrial disease research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, while also offering sourcing for rare target raw materials to accelerate your development pipeline.