Anti-ALDH3A1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P11883

Core Product Specifications and Parameters

Biological Background: ALDH3A1 Function and Localization

• Aldehyde dehydrogenase family 3 member A1 (ALDH3A1) is a cytoplasmic enzyme belonging to the aldehyde dehydrogenase (ALDH) superfamily. It is also known as HTC-ALDH or tumor-associated aldehyde dehydrogenase.
• ALDH3A1 preferentially oxidizes aromatic aldehyde substrates and also acts on medium and long chain aldehydes, converting them into non-toxic fatty acids. Related references: PMID:2831537
• ALDHs, including ALDH3A1, are involved in the detoxification of alcohol-derived acetaldehyde and play roles in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation products.
• In the cornea, ALDH3A1 constitutes about 50% of the soluble protein in epithelial cells and is thought to protect against UV-induced damage.
• The protein is a dimeric, NADP-preferring oxidoreductase. Post-translational modifications include acetylation.
• The gene Aldh3a1 (also known as Aldd, Aldh3) encodes this protein in rat.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-103 at the N-terminus of rat ALDH3A1. This region is relatively conserved among human, mouse, and rat, which may contribute to the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is approximately 50 kDa. Consider using a gel percentage appropriate for this size range and validate with a suitable loading control in your target tissue or cell line.
• For IHC and IF applications, corneal epithelium expresses high levels of ALDH3A1 and can serve as a positive control tissue. Optimize antigen retrieval and antibody dilution conditions as needed.
• In ELISA, ensure that the coating antigen or capture format is compatible with the antibody's binding characteristics. Pairing with a detection antibody should be evaluated for sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is committed to providing diagnostic manufacturers, laboratories, and research institutes with reliable one-stop access to IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for ALDH family targets, supporting aldehyde-related disease research and assay development. Contact our team for custom sourcing and technical consultation tailored to your project needs.