Anti-ALDH3A1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P30838

Core Product Specifications and Parameters

Biological Background: ALDH3A1 Function and Localization

• ALDH3A1 (Aldehyde dehydrogenase, dimeric NADP-preferring) is a member of the aldehyde dehydrogenase family, also known as ALDHIII or aldehyde dehydrogenase 3. It is encoded by the ALDH3A1 gene in humans.
• It plays a major role in detoxifying alcohol-derived acetaldehyde and is involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable).
• ALDH3A1 oxidizes medium and long chain aldehydes into non-toxic fatty acids and preferentially oxidizes aromatic aldehyde substrates. Related references: PMID:1737758
• It constitutes about 50% of corneal epithelial soluble proteins and may help protect the cornea from UV-induced damage (By similarity).
• The protein localizes to the cytoplasm and is highly expressed in stomach, esophagus, and lung, with lower levels in liver and kidney.
• Keywords associated with ALDH3A1 include acetylation, lipid metabolism, NAD/NADP dependence, oxidoreductase activity, and 3D-structure availability.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-300 of human ALDH3A1 (NP_001128640.1). Consider using this sequence information to verify epitope recognition or for competition assays.
• For Western blot (WB), the expected band is approximately 50kDa; include positive and negative controls from relevant tissues (e.g., cornea, stomach) to confirm specificity.
• In immunofluorescence (IF/ICC or IF-P), ALDH3A1 exhibits cytoplasmic staining; ensure proper permeabilization and use appropriate fixation methods.
• ELISA performance may be optimized by testing different coating antigen concentrations and comparing with known standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. With expertise in aldehyde dehydrogenase targets and corneal biology research, CamelBio supports diagnostic developers from concept to clinic.