Anti-ALDH2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05091

Core Product Specifications and Parameters

Biological Background: ALDH2 Function and Localization

• ALDH2 (Aldehyde dehydrogenase, mitochondrial) is also known as ALDH class 2, ALDH-E2, and ALDHI, and is encoded by the ALDH2 gene.
• The enzyme localizes to the mitochondrial matrix, where it functions as a key oxidoreductase using NAD as a cofactor.
• Its primary role is the detoxification of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that can induce DNA damage.
• ALDH2 is subject to post-translational modifications including acetylation and ubiquitylation, which may regulate its activity and stability.
• Alternative splicing generates multiple transcript variants, and genetic mutations in ALDH2 are linked to disease variants associated with alcohol metabolism disorders, dwarfism, and intellectual disability.
• The protein has a calculated molecular weight of approximately 56 kDa and has been identified through direct protein sequencing and mass spectrometry-based proteomics.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 321-480 of human ALDH2, a region likely to generate antibodies that recognize both the native and denatured protein in multiple applications.
• For western blotting (WB), the predicted band size is approximately 56 kDa; consider using a reducing loading buffer and validate in human, mouse, or rat tissue lysates known to express ALDH2 (e.g., liver, kidney).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilution; positive controls may include human liver or kidney sections.
• ELISA: This antibody can be used as a capture or detection reagent; validate specificity with recombinant ALDH2 protein or known positive samples.
• As a polyclonal antibody, batch-to-batch consistency should be verified if employed in quantitative assays or IVD kit development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your partner for one-stop IVD raw-material sourcing, providing access to validated antibodies, bulk ancillary reagents, and rare-target raw materials tailored for ALDH2 research. Whether you are developing assays for alcohol metabolism, toxicology, or cancer biomarker studies, CamelBio can supply optimized monoclonal and polyclonal antibodies, antibody pairs, and related reagents to streamline your path from concept to clinic. We bridge the gap between research and diagnostic manufacturing, ensuring consistent quality and scalable supply.