Anti-ALDH2 Polyclonal Antibody for WB, ELISA - P05091

Core Product Specifications and Parameters

Biological Background: ALDH2 Function and Localization

• ALDH2 encodes mitochondrial aldehyde dehydrogenase, a key enzyme belonging to the ALDH class 2 family.
• It is essential for the clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage.
• The protein localizes to the mitochondrial matrix, consistent with its role in detoxifying metabolic aldehydes.
• ALDH2 is known to undergo post-translational modifications, including acetylation and ubiquitin conjugation, as indicated by proteomics studies.
• Alternative splicing produces multiple isoforms of ALDH2, and mutations in the gene are associated with diseases such as dwarfism and intellectual disability (as per UniProt keywords).
• The enzyme requires NAD as a cofactor for its oxidoreductase activity, confirmed by direct protein sequencing and 3D structural data.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-380) of human ALDH2, which is highly conserved across species, supporting its cross-reactivity with mouse and rat orthologs.
• For Western blotting, an expected band at approximately 56 kDa can be visualized using standard SDS-PAGE under reducing conditions.
• ALDH2 is abundantly expressed in liver and kidney mitochondria; consider using these tissues as positive controls for validation.
• For ELISA applications, the polyclonal antibody may detect both native and denatured ALDH2; optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions for metabolic enzyme research, including validated antibodies like this anti-ALDH2 polyclonal antibody. From monoclonal and polyclonal antibody pairs to bulk ancillary reagents, we support diagnostic developers and laboratories in sourcing rare-target materials and optimizing assay workflows. Partner with CamelBio to bridge the gap from concept to clinic with reliable, high-quality reagents.