Anti-ALDH1L2 Polyclonal Antibody for WB and ELISA - Q3SY69

Core Product Specifications and Parameters

Biological Background: ALDH1L2 Function and Localization

• ALDH1L2 (also known as mtFDH or aldehyde dehydrogenase family 1 member L2) is a mitochondrial 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide, playing a key role in one-carbon metabolism.
• It belongs to the aldehyde dehydrogenase family and is localized to the mitochondrion, consistent with its involvement in mitochondrial folate metabolism.
• The protein is highly expressed in pancreas, heart, brain, and skeletal muscle, suggesting tissue-specific regulatory roles in these organs.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains a phosphopantetheine moiety.
• Alternative splicing generates multiple isoforms, which may have distinct functional properties.
• The molecular weight calculated from the sequence is approximately 101,746 Da (predicted ~102 kDa on SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280-480 of human ALDH1L2, a region likely conserved across species; reported cross-reactivity with mouse and rat. Validate specificity in your target species and sample type.
• For WB, the predicted molecular weight is 102 kDa. Use appropriate molecular weight markers and confirm band identity with positive control lysates, e.g., from pancreas or heart.
• ALDH1L2 is highly expressed in pancreas, heart, brain, and skeletal muscle; consider these tissues as positive controls for both WB and ELISA applications.
• As a polyclonal antibody, lot-to-lot variability may occur. Optimize dilution empirically for each new lot, and include internal reference standards to ensure consistency in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, from concept to clinic. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials. For mitochondrial enzyme targets like ALDH1L2 in one-carbon metabolism, we support diagnostic manufacturers and research laboratories with reliable, high-quality reagents tailored to your assay development needs.