Anti-ALDH1L1 Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - O75891

Core Product Specifications and Parameters

Biological Background: ALDH1L1 Function and Localization

• ALDH1L1 encodes cytosolic 10-formyltetrahydrofolate dehydrogenase, which catalyzes the NADP+-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide, playing a central role in one-carbon metabolism. Related references: PMID:19933275 PMID:21238436
• The enzyme has been reported to possess additional NADP+-dependent aldehyde dehydrogenase activity towards substrates including formaldehyde, acetaldehyde, and benzaldehyde (By similarity).
• ALDH1L1 is localized to the cytoplasm and cytosol, consistent with its metabolic functions.
• It is highly expressed in liver, pancreas, and kidney, suggesting tissue-specific roles in folate metabolism.
• Post-translational modifications include acetylation and phosphorylation; the protein also harbors a phosphopantetheine moiety, a hallmark of acyl carrier domains.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity (Keywords: Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen (aa 180-300) lies within a region predicted to be surface-accessible, which may facilitate recognition of the native ALDH1L1 protein in immunoassays.
• In western blotting, a specific band at approximately 99 kDa is expected; use appropriate molecular weight markers for verification.
• For immunofluorescence applications, consider cytoplasmic localization and optimize permeabilization steps accordingly.
• When performing immunohistochemistry on paraffin-embedded tissues, antigen retrieval using heat-induced epitope retrieval (HIER) in citrate or EDTA buffer is recommended.
• For ELISA, direct coating with recombinant ALDH1L1 or the immunogen peptide is suggested for antibody capture assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, offering this ALDH1L1 rabbit monoclonal antibody along with a broad portfolio of validated antibodies, optimized antigen pairs, and ancillary reagents. Whether your research focuses on one-carbon metabolism, cancer biology, or tissue-specific enzyme functions, we streamline procurement from concept to clinic. Our supply capabilities include bulk orders, custom monoclonal development, and rare-target sourcing to accelerate your diagnostic development timelines.