Anti-ALDH1L1 Polyclonal Antibody for WB, ELISA - O75891

Core Product Specifications and Parameters

Biological Background: ALDH1L1 Function and Localization

• ALDH1L1 (Aldehyde dehydrogenase family 1 member L1), also known as FDH, FTHFD, 10-fTHF, is a cytosolic enzyme belonging to the aldehyde dehydrogenase family.
• Catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide, a key reaction in folate-mediated one-carbon metabolism. Related references: PMID:19933275 PMID:21238436
• May also exhibit aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (by similarity).
• Subcellular localization: cytoplasm, cytosol.
• Tissue specificity: highly expressed in liver, pancreas, and kidney.
• Post-translational modifications include acetylation and phosphopantetheine addition; it is also a phosphoprotein.
• Keywords: One-carbon metabolism, NADP, oxidoreductase, phosphopantetheine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-250 of human ALDH1L1, covering the N-terminal region; therefore, the antibody may recognize both full-length and truncated isoforms.
• For Western blotting, a band around 99 kDa is predicted; validate size using appropriate molecular weight markers and consider that post-translational modifications may affect migration.
• The antibody is suitable for ELISA; perform optimization of coating antigens and detection conditions for best results.
• Cross-reactivity with mouse and rat has been confirmed; verify performance in your specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, providing diagnostic developers and researchers with validated antibodies, recombinant proteins, and ancillary reagents. This anti-ALDH1L1 polyclonal antibody supports studies in folate metabolism and one-carbon pathways. For bulk, customized, or rare target sourcing, CamelBio ensures reliable supply chain integration from concept to clinic.