Anti-ALDH1B1 Polyclonal Antibody for WB, IF/ICC, ELISA - P30837

Core Product Specifications and Parameters

Biological Background: ALDH1B1 Function and Localization

• ALDH1B1 (aldehyde dehydrogenase family 1 member B1), also known as ALDH5 or ALDHX, is a mitochondrial aldehyde dehydrogenase encoded by the ALDH1B1 gene. It belongs to the ALDH superfamily, which plays critical roles in detoxifying endogenous and exogenous aldehydes.
• The enzyme is a broad-specificity aldehyde dehydrogenase that detoxifies alcohol-derived acetaldehyde, and other aldehydes, with high catalytic efficiency toward aliphatic medium-chain saturated aldehydes.
  Related references: PMID:20616185, PMID:25413692, PMID:35788181
• It can also metabolize products of lipid peroxidation, namely 4-hydroxynon-2-enal (4-HNE) and malonaldehyde (MDA), albeit with lower catalytic efficiency.
  Related references: PMID:20616185
• ALDH1B1 shows an absolute preference for NAD(+) as cofactor; no activity is detected with NADP(+).
  Related references: PMID:20616185, PMID:25413692
• In vitro, the enzyme displays nitroglycerin reductase activity and esterase activity, though the physiological relevance of the esterase function is uncertain.
  Related references: PMID:25413692, PMID:20616185
• ALDH1B1 localizes specifically to the mitochondrial matrix, as indicated by its transit peptide.
• It is predominantly expressed in liver and testis, with lower levels in the brain.
• The protein has a molecular weight of approximately 57 kDa, consistent with the calculated mass of 57,249 Da, and undergoes post-translational modifications including acetylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 278–517) corresponds to the C-terminal region of human ALDH1B1. This region may be suitable for detecting both full-length and potential truncated forms; consider validating in samples known to express ALDH1B1, such as liver or testis lysates.
• For Western blot, anticipate a band around 57 kDa. Validate the antibody's sensitivity and specificity by including positive and negative controls, and consider loading appropriate subcellular fractions to confirm mitochondrial localization.
• In immunofluorescence/ICC, use mitochondrial counterstains (e.g., MitoTracker) to co-localize ALDH1B1 signals. For ELISA, the antibody can be employed for quantitative detection of recombinant or overexpressed ALDH1B1; optimize coating and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we are your one-stop sourcing bridge for IVD raw materials, providing reliable, optimized antibodies like this anti-ALDH1B1 polyclonal antibody to support research in alcohol metabolism, oxidative stress, and mitochondrial disorders. Our broad supply capabilities include validated antibody pairs, bulk ancillary reagents, and rare target raw-material procurement, streamlining your diagnostic development from concept to clinic. Partner with us to access the tools necessary for advancing aldehyde-related diagnostics and therapeutic research.