Anti-ALDH1B1 Polyclonal Antibody for WB, IF/ICC, ELISA - P30837

Core Product Specifications and Parameters

Biological Background: ALDH1B1 Function and Localization

• ALDH1B1 (aldehyde dehydrogenase family 1 member B1, mitochondrial), also known as ALDH5, ALDHX, or nitroglycerin reductase, may contribute to the detoxification of alcohol-derived acetaldehyde and other aldehydes. Related references: PMID:20616185 PMID:25413692 PMID:35788181
• The enzyme demonstrates broad substrate specificity, preferentially oxidizing aliphatic medium-chain saturated aldehydes; it also metabolizes lipid peroxidation products 4-HNE and MDA, and exhibits in vitro nitroglycerin reductase activity. Related references: PMID:20616185 PMID:25413692
• ALDH1B1 shows an absolute preference for NAD(+) as a cofactor, with no detectable activity using NADP(+). Related references: PMID:20616185 PMID:25413692
• Subcellular localization is mitochondrial, within the matrix.
• Expression is highest in liver and testis, with lower levels in brain.
• The protein undergoes acetylation, contains a mitochondrial transit peptide, and its 3D structure has been determined; it is cataloged in the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 278-517) of human ALDH1B1; therefore, the antibody is expected to recognize full-length or C-terminal fragments.
• For Western blot, consider analyzing mitochondrial-enriched fractions from human liver or testis samples, where ALDH1B1 is abundantly expressed, to confirm the ~57 kDa band.
• In immunofluorescence and immunocytochemistry, mitochondrial staining is anticipated; co-staining with a mitochondrial marker is recommended to verify subcellular localization.
• As a polyclonal antibody, performance may vary between lots; validate each new lot in the relevant assay system before use.
• ELISA optimization may require testing different coating antigen concentrations and antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined sourcing portal for high-quality IVD raw materials. Complementing our ALDH1B1 antibody, we offer validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibody development to accelerate your aldehyde dehydrogenase research and diagnostic assay development. Leverage our integrated supply chain for rare target raw materials and technical support from concept to clinic.