Anti-ALDH1A2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O94788

Core Product Specifications and Parameters

Biological Background: ALDH1A2 Function and Localization

• ALDH1A2 encodes retinal dehydrogenase 2 (RALDH2), a key NAD-dependent enzyme that oxidizes all-trans-retinal and all-trans-13,14-dihydroretinal to the corresponding retinoic acids, which are critical signaling molecules for transcriptional regulation (PMID:29240402, PMID:33565183). Related references: PMID:29240402 PMID:33565183
• Retinoate signaling initiated by ALDH1A2 is essential for transcriptional control of numerous genes and is required for the initiation of meiosis in both male and female germ cells (PMID:33565183). Related references: PMID:33565183
• ALDH1A2 can metabolize octanal and decanal but shows very low activity toward benzaldehyde, acetaldehyde, or propanal, and completely lacks activity with citral, indicating its high substrate specificity for retinoids.
• The protein localizes to the cytoplasm where it encounters free and CRBP-bound retinal substrates.
• Post-translational modifications include phosphorylation, and the gene produces multiple isoforms via alternative splicing.
• Clinically, mutations in ALDH1A2 are associated with disease variants, underscoring its importance in normal physiology and pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (residues 1-180), which is predicted to be present in major isoforms; consider verifying isoform detection in your specific model system.
• For WB, expected band around 57 kDa; additional bands may appear due to alternative splicing or post-translational modification—validate with appropriate controls.
• Cross-reactivity with mouse and rat is specified; confirm reactivity in your species of interest using positive control lysates before large-scale experiments.
• For IHC-P and IF/ICC, antigen retrieval and permeabilization steps may need optimization; include appropriate tissue or cell controls.
• ELISA applications may benefit from pairing with a detection antibody targeting a distant epitope; conduct pilot assays to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This ALDH1A2 antibody, targeting a crucial enzyme in retinoic acid signaling, supports diagnostic and research applications in developmental biology, oncology, and infertility studies. Leverage CamelBio's one-stop service to accelerate your concept-to-clinic pipeline with reliable, high-quality raw materials.