Anti-ALDH1A1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00352

Core Product Specifications and Parameters

Biological Background: ALDH1A1 Function and Localization

• ALDH1A1 (Aldehyde dehydrogenase 1A1) is a member of the aldehyde dehydrogenase family, encoded by the ALDH1A1 gene (also known as ALDC, ALDH1, PUMB1). It is a cytosolic NAD-dependent enzyme.
• Functions as a retinal dehydrogenase, catalyzing the oxidation of retinaldehyde to retinoic acid, a key step in vitamin A metabolism and retinoic acid signaling. Related references: PMID:25413692
• Plays a detoxifying role by oxidizing various aldehydes derived from lipid peroxidation, such as 4-hydroxynonenal (HNE) and malonaldehyde, thereby preventing protein adduct formation and cellular damage. Related references: PMID:12941160, PMID:15623782, PMID:19296407
• Participates in the fructosamine degradation pathway by oxidizing 3-deoxyglucosone, a potent glycating agent, thus reducing protein glycation. Related references: PMID:17175089
• Additionally, exhibits aminobutyraldehyde dehydrogenase activity, possibly contributing to GABA biosynthesis in the midbrain.
• Subcellular localization includes cytoplasm, cytosol, and axon projections, indicating roles in both somatic and neuronal compartments.
• Expressed in erythrocytes at the protein level; post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 301-400 of human ALDH1A1; consider sequence alignment to confirm expected cross-reactivity with mouse and rat orthologs.
• For Western blot (WB), a predominant band around 55 kDa is anticipated; validate in the relevant cell or tissue lysates.
• For IHC-P and IF/ICC, antigen retrieval may be necessary; optimize fixation and permeabilization conditions for the specific sample type.
• ELISA applications may require antibody titration and the use of pre-immune serum as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This ALDH1A1 rabbit polyclonal antibody supports research in aldehyde metabolism, retinoic acid signaling, and oxidative stress. Explore CamelBio's sourcing capabilities for rare targets and custom antibody development to accelerate your diagnostic projects.