Anti-ALDH1A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00352

Core Product Specifications and Parameters

Biological Background: ALDH1A1 Function and Localization

• Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1 (RALDH1), is a cytosolic enzyme belonging to the aldehyde dehydrogenase family.
• Catalyzes the oxidation of retinaldehyde to retinoic acid, a key step in vitamin A metabolism and gene regulation (PubMed:25413692). Related references: PMID:25413692
• Detoxifies lipid peroxidation-derived aldehydes, such as (E)-4-hydroxynon-2-enal (HNE), preventing protein adduct formation and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Related references: PMID:12941160, PMID:15623782, PMID:19296407
• Participates in the fructosamine degradation pathway by oxidizing 3-deoxyglucosone, reducing protein glycation (PubMed:17175089). Related references: PMID:17175089
• Possesses aminobutyraldehyde dehydrogenase activity and may contribute to GABA biosynthesis in the midbrain (By similarity).
• Localizes predominantly to the cytoplasm and can extend into cell projections such as axons.
• Expressed at the protein level in erythrocytes; subject to acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 250-350 of human ALDH1A1, suggesting epitope specificity to this region. For Western blot (WB), expect a band ~55 kDa; include a suitable loading control.
• For IHC-P and IF/ICC, optimize antigen retrieval conditions per sample type and validate in ALDH1A1-expressing tissues such as lens epithelium.
• This antibody is reactive with human and rat samples. If using other species, align the immunogen sequence to predict cross-reactivity.
• ELISA applications may require optimization of coating conditions and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies like this ALDH1A1 rabbit mAb, bulk ancillary reagents, and rare-target procurement. We support research in retinoic acid metabolism and aldehyde detoxification, assisting with everything from early assay development to clinical validation. Reach out to our team for tailored sourcing and technical consultation.