Anti-ALDH1A1 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P00352

Core Product Specifications and Parameters

Biological Background: ALDH1A1 Function and Localization

• ALDH1A1, also known as ALDC, RALDH1, and ALDH-E1, is a member of the aldehyde dehydrogenase family and exists as a cytosolic enzyme.
• It catalyzes the NAD-dependent oxidation of various aldehydes to carboxylic acids, playing a key role in detoxification and metabolism (PubMed:12941160, PubMed:15623782, PubMed:17175089).
  Related references: PMID:12941160, PMID:15623782, PMID:17175089
• A critical function is the oxidation of retinaldehyde to retinoic acid, the second step in vitamin A metabolism, essential for controlling retinol and retinoic acid levels (PubMed:25413692).
  Related references: PMID:25413692
• It detoxifies lipid peroxidation products like 4-hydroxynon-2-enal (HNE), malonaldehyde, and hexanal, thereby preventing the formation of cytotoxic protein adducts and contributing to lens clarity (PubMed:12941160, PubMed:15623782, PubMed:19296407).
  Related references: PMID:12941160, PMID:15623782, PMID:19296407
• ALDH1A1 participates in fructosamine degradation by oxidizing 3-deoxyglucosone, a potent glycating agent, thus helping to reduce protein glycation (PubMed:17175089).
  Related references: PMID:17175089
• The enzyme may also function in an alternative GABA biosynthesis pathway in the midbrain through aminobutyraldehyde dehydrogenase activity (by similarity).
• Subcellularly, ALDH1A1 is primarily localized to the cytoplasm and cytosol, and has been detected in axon projections.
• It is expressed in erythrocytes at the protein level, and subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide, suggesting it likely recognizes a linear epitope; therefore, it should perform well in denaturing western blot (WB) applications.
• For WB, expect a specific band around 55 kDa; validate against appropriate positive and negative controls to confirm specificity in your sample system.
• In immunofluorescence (IF/ICC), anticipate cytoplasmic and possibly axonal staining patterns; include relevant cell or tissue controls.
• For immunoprecipitation (IP) and ELISA applications, consider optimizing antibody concentration and buffer conditions to achieve optimal binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one‑stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high‑quality reagents like this ALDH1A1 antibody for studies in retinoid signaling, oxidative stress, and metabolic detoxification. Our extensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.