Anti-ALDH1A1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00352

Core Product Specifications and Parameters

Biological Background: ALDH1A1 Function and Localization

• Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1 (RALDH1), ALDH1, ALDC, PUMB1, or HEL-S-53e, is a cytosolic enzyme belonging to the aldehyde dehydrogenase family.
• Functions as a key enzyme in retinoic acid biosynthesis by catalyzing the irreversible oxidation of retinaldehyde to retinoic acid, downstream of retinol dehydrogenases (PubMed:25413692). This pathway is crucial for controlling teratogenic and cytotoxic levels of retinol and retinoic acid (PubMed:25413692). Related references: PMID:25413692
• Also oxidizes aldehydes from lipid peroxidation, such as (E)-4-hydroxynon-2-enal (HNE), malonaldehyde, and hexanal, thereby preventing protein adduct formation and lens opacification (PubMed:12941160, PubMed:15623782, PubMed:19296407). Related references: PMID:12941160, PMID:15623782, PMID:19296407
• Participates in the fructosamine degradation pathway by oxidizing 3-deoxyglucosone, a potent glycating agent (PubMed:17175089). Related references: PMID:17175089
• Exhibits aminobutyraldehyde dehydrogenase activity, potentially contributing to GABA biosynthesis in the midbrain (By similarity).
• Localizes primarily to the cytoplasm, cytosol, and cell projections such as axons.
• Expressed at the protein level in erythrocytes.
• Post-translationally modified by acetylation and phosphorylation; annotated with keywords including NAD binding, nucleotide-binding, and oxidoreductase activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 250-350 of human ALDH1A1; consider epitope accessibility in native conformation and perform appropriate antigen retrieval for IHC-P.
• Predicted molecular weight is 55kDa; validate the band in WB using relevant positive control lysates (e.g., human liver or retinal tissue).
• The antibody cross-reacts with mouse and rat; verify reactivity in your target species by sequence alignment of the immunogen region if needed.
• For IF/ICC, optimize fixation and permeabilization conditions to preserve the cytoplasmic localization signal.
• Suitable for ELISA; consider testing in a sandwich format with an appropriate paired antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide one-stop access to IVD raw materials from concept to clinic. This high-quality anti-ALDH1A1 monoclonal antibody supports research in stem cell biology, cancer diagnostics, and retinoid metabolism. We offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents, ensuring reliable and scalable supply for your assay development needs.