Anti-ALDH18A1 Rabbit Polyclonal Antibody for WB, ELISA - P54886

Core Product Specifications and Parameters

Biological Background: ALDH18A1 Function and Localization

• ALDH18A1, also known as delta-1-pyrroline-5-carboxylate synthase (P5CS), is a bifunctional enzyme belonging to the aldehyde dehydrogenase family.
• It catalyzes the conversion of glutamate to glutamate 5-semialdehyde, a critical intermediate in the biosynthesis of proline, ornithine, and arginine.
• The enzyme contains both kinase and glutamyl phosphate reductase activities, requiring ATP and NADP as cofactors.
• Localized to the mitochondrial matrix, ALDH18A1 plays a central role in mitochondrial amino acid metabolism.
• Alternative splicing generates multiple isoforms, which may exhibit tissue-specific expression patterns.
• Mutations in ALDH18A1 are associated with neurological disorders, including hereditary spastic paraplegia and neurodegeneration.
• The protein is also implicated in intellectual disability and cutis laxa syndromes, underscoring its importance in development and neuroprotection.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-240), which may contain conserved domains across species; verify cross-reactivity in your experimental system.
• For Western blot, the predicted molecular weight is approximately 87 kDa; use appropriate gel percentage and transfer conditions.
• In ELISA, consider optimizing coating conditions and antibody dilution based on the target protein concentration.
• Since ALDH18A1 is mitochondrial, sample preparation may require mitochondrial enrichment or subcellular fractionation for optimal detection.
• This polyclonal antibody may recognize multiple isoforms due to alternative splicing; confirm specificity using knockdown or overexpression controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our anti-ALDH18A1 antibody supports research in amino acid metabolism, mitochondrial disorders, and neurological disease biomarkers. As your sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.