Anti-ALDH16A1 Monoclonal Antibody for WB, ELISA - Q8IZ83

Core Product Specifications and Parameters

Biological Background: ALDH16A1 Function and Localization

• ALDH16A1 (aldehyde dehydrogenase family 16 member A1) is a protein encoded by the ALDH16A1 gene (GeneID: 126133) in humans.
• It belongs to the aldehyde dehydrogenase superfamily, which catalyzes the NAD(P)+-dependent oxidation of aldehydes to carboxylic acids, playing roles in detoxification and metabolism.
• Alternative splicing of the ALDH16A1 transcript produces multiple isoforms, as indicated by the keyword "Alternative splicing."
• The protein has been detected in proteomics analyses, suggesting expression in various tissues (keyword: "Proteomics identification"), and is included in the UniProt human reference proteome.
• The calculated molecular mass of the canonical isoform is approximately 85 kDa (85,127 Da), consistent with the observed weight of 85kDa in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 500–600), so the antibody is likely to recognize an epitope in that domain. Consider using this property for epitope mapping or domain-specific detection.
• Validated applications include Western blot (WB) and ELISA. For WB, a band of ~85 kDa is expected. Optimize loading and transfer conditions to resolve high-molecular-weight proteins.
• The antibody shows cross-reactivity with mouse and rat orthologs, making it suitable for studies across these species. Validate specificity in your sample system by including positive and negative controls.
• For ELISA, determine optimal coating antigen and antibody dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing, including validated monoclonal antibodies like this ALDH16A1 Rabbit mAb. Our platform streamlines procurement for diagnostic developers and researchers, offering access to bulk quantities, technical support, and ancillary reagents tailored to your assay development pipelines.