Anti-Alcohol Dehydrogenase Rabbit Polyclonal Antibody for WB, ELISA - P07327

Core Product Specifications and Parameters

Biological Background: Alcohol Dehydrogenase 1A Function and Localization

• ADH1A encodes the alpha subunit of alcohol dehydrogenase, a member of the alcohol dehydrogenase family.
• The enzyme functions as a homodimer and oxidizes primary and secondary alcohols, although ethanol is a very poor substrate. Related references: PMID:2738060
• ADH1A is localized in the cytoplasm.
• It contains binding sites for zinc and NAD, and undergoes post-translational modifications including acetylation and phosphorylation.
• The protein has been identified through direct protein sequencing and proteomics.
• The predicted molecular weight is approximately 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 220-300) of human ADH1A, which may be conserved in rat, supporting observed cross-reactivity.
• For Western blot (WB), a band at ~40 kDa is expected; consider using 20-30 µg of whole cell lysate and validate in the relevant sample system.
• For ELISA, titrate the antibody against recombinant or endogenous ADH1A to determine the optimal working concentration.
• The antibody is supplied as a rabbit polyclonal preparation; note that polyclonal antibodies may exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our portfolio of Alcohol Dehydrogenase (ADH1A)-related reagents supports alcohol metabolism research and assay development, complemented by expert technical consulting. Let CamelBio be your bridge from concept to clinic.